Biopolym. Cell. 1989; 5(3):102-109.
Структура та функції біополімерів
Незалежна візуалізація рецепторів до нативних і модифікованих ліпопротеїнів низької щільності на клітинах в культурі
- Інститут експериментальної кардіології, Всесоюзного кардіологічного наукового центру АМН СРСР
Москва, СРСР
Abstract
Запропоновано метод, що дозволяє незалежно візуалізувати рецептори до нативних і модифікованих ліпопротеїнів низької щільності на клітинах у культурі. В основі методу – реконструкція ліпопротеїнів холестериловими ефірами, що містять різні хромофори, рецепторне зв’язування їх з клітинами, флуоресцентна мікроскопія клітин за різних довжин хвиль.
Повний текст: (PDF, російською)
References
[1]
Brown MS, Goldstein JL. Receptor-mediated endocytosis: insights from the lipoprotein receptor system. Proc Natl Acad Sci U S A. 1979;76(7):3330-7.
[2]
Mahley RW, Innerarity TL. Lipoprotein receptors and cholesterol homeostasis. Biochim Biophys Acta. 1983;737(2):197-222.
[3]
Goldstein JL, Brown MS. Binding and degradation of low density lipoproteins by cultured human fibroblasts. Comparison of cells from a normal subject and from a patient with homozygous familial hypercholesterolemia. J Biol Chem. 1974;249(16):5153-62.
[4]
Henriksen T, Mahoney EM, Steinberg D. Enhanced macrophage degradation of low density lipoprotein previously incubated with cultured endothelial cells: recognition by receptors for acetylated low density lipoproteins. Proc Natl Acad Sci U S A. 1981;78(10):6499-503.
[5]
Pitas RE, Innerarity TL, Mahley RW. Foam cells in explants of atherosclerotic rabbit aortas have receptors for beta-very low density lipoproteins and modified low density lipoproteins. Arteriosclerosis. 1983;3(1):2-12.
[6]
Goldstein JL, Ho YK, Basu SK, Brown MS. Binding site on macrophages that mediates uptake and degradation of acetylated low density lipoprotein, producing massive cholesterol deposition. Proc Natl Acad Sci U S A. 1979;76(1):333-7.
[7]
Mahley RW, Innerarity TL, Weisgraber KB, Oh SY. Altered metabolism (in vivo and in vitro) of plasma lipoproteins after selective chemical modification of lysine residues of the apoproteins. J Clin Invest. 1979;64(3):743-50.
[8]
Fogelman AM, Shechter I, Seager J, Hokom M, Child JS, Edwards PA. Malondialdehyde alteration of low density lipoproteins leads to cholesteryl ester accumulation in human monocyte-macrophages. Proc Natl Acad Sci U S A. 1980;77(4):2214-8.
[9]
Brown MS, Goldstein JL. Lipoprotein metabolism in the macrophage: implications for cholesterol deposition in atherosclerosis. Annu Rev Biochem. 1983;52:223-61.
[10]
Sklar LA, Mantulin WW, Pownall HJ. Fluorescent cholesteryl esters in the core of low density lipoprotein. Biochem Biophys Res Commun. 1982;105(2):674-80.
[11]
Kao YJ, Soutar AK, Hong KY, Pownall HJ, Smith LC. N-(2-Naphthyl)-23,24-dinor-5-cholen-22-amin-3beta-ol, a fluorescent cholesterol analogue. Biochemistry. 1978;17(13):2689-96.
[12]
Krieger M, Smith LC, Anderson RG, Goldstein JL, Kao YJ, Pownall HJ, Gotto AM Jr, Brown MS. Reconstituted low density lipoprotein: a vehicle for the delivery of hydrophobic fluorescent probes to cells. J Supramol Struct. 1979;10(4):467-78.
[13]
Falck JR, Krieger M, Goldstein JL, Brown MS. Preparation and spectral properties of lipophilic fluorescein derivatives: application to plasma low-density lipoprotein. J Am Chem Soc. 1981;103(24):7396–8.
[14]
Craig IF, Via DP, Mantulin WW, Pownall HJ, Gotto AM Jr, Smith LC. Low density lipoproteins reconstituted with steroids containing the nitrobenzoxadiazole fluorophore. J Lipid Res. 1981;22(4):687-96.
[15]
Krieger M, Brown MS, Faust JR, Goldstein JL. Replacement of endogenous cholesteryl esters of low density lipoprotein with exogenous cholesteryl linoleate. Reconstitution of a biologically active lipoprotein particle. J Biol Chem. 1978;253(12):4093-101.
[16]
Sklar LA, Craig IF, Pownall HJ. Induced circular dichroism of incorporated fluorescent cholesteryl esters and polar lipids as a probe of human serum low density lipoprotein structure and melting. J Biol Chem. 1981;256(9):4286-92.
[17]
Patsch JR, Sailer S, Kostner G, Sandhofer F, Holasek A, Braunsteiner H. Separation of the main lipoprotein density classes from human plasma by rate-zonal ultracentrifugation. J Lipid Res. 1974;15(4):356-66.
[18]
Lindgren FT. Preparative ultracentrifugal laboratory procedures and suggestions for lipoprotein analysis. Analysis lipids and lipoproteins. Ed. E. C. Perkins. New York: Amer. Oil Chemists Soc., 1975:204-224.
[19]
Haberland ME, Fogelman AM, Edwards PA. Specificity of receptor-mediated recognition of malondialdehyde-modified low density lipoproteins. Proc Natl Acad Sci U S A. 1982;79(6):1712-6.
[20]
Lowry OH, Rosebrough NJ, Farr AL, RANDALL RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951;193(1):265-75.
[21]
Preobrazhensky SN, Ivanov VO, Fuki IV, Tsibulsky VP, Kameneva AM, Repin VS, Ermolin GA. Enzyme-linked immunoreceptor assay of low-density-lipoprotein receptors. Anal Biochem. 1985;149(1):269-74.
[22]
Vaskovsky VE, Kostetsky EY, Vasendin IM. A universal reagent for phospholipid analysis. J Chromatogr. 1975;114(1):129-41.
[23]
Vladimirov YuA, Dobretsov GE. Fluorescent Probes in the study of biological membranes. Moskow, Nauka, 1980; 320 P.
[24]
Preobrazhensky SN, Tsibulsky VP, Fuki IV, Ivanov VO, Repin VS, Smirnov VN. Enzyme immunoassay of the receptors for modified low density lipoprotein. Anal Biochem. 1986;154(2):382-7.
[25]
A. c. USSR 1126574, MKI4, H 1 February. 08. Process for Producing sPin-labeled cholesteryl ester, and sPin-labeled lecithin. AYu Misharin, NG Bushmakina, BK Chernov. Otkrytiya. Izobreteniya. 1984; 44:109-111.
[26]
Lehrer SS. Solute perturbation of protein fluorescence. The quenching of the tryptophyl fluorescence of model compounds and of lysozyme by iodide ion. Biochemistry. 1971;10(17):3254-63.