Biopolym. Cell. 1989; 5(2):47-51.
http://dx.doi.org/10.7124/bc.0000A5
Дискусії
Можливість експресії клонованого гена еритропоетину людину в клітинах лінії СНО
1Крамєрова І. А., 1Зеленін М. Г., 1Воронцова М. М., 1Колобков С. Л., 1Монакова Т. Є.
  1. НДІ біомедичних технології МОЗ СРСР
    Москва, СРСР

Abstract

Раніше нами проведено скринінг геномної бібліотеки ДНК людини і ідентифіковано рекомбінантний фаг, що містить ген еритропоетину людини – основного фактора диференціювання клітин еритроїдного ряду. Отримано плазміду рSVEро, що містить ген еритропоетину під контролем промотору ранніх генів вірусу SV40. У культуральній рідині клітин лінії СНО, трансфекованих плазмідою рSVEро, виявлено еритропоетичну активність. Плазміда рSVEро може бути використана в експериментах з генотерапії соматичних клітин.
Повний текст: (PDF, російською)

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