Possibility of the expression of a cloned human erythropoietin gene in CHO cells

Kramerova, I. A.; Zelenin, M. G.; Vorontsova, M. M.; Kolobkov, S. L.; Monakova, T. E.

doi:10.7124/bc.0000A5

Biopolym. Cell. 1989; 5(2):47-51.

http://dx.doi.org/10.7124/bc.0000A5

Discussions

Possibility of the expression of a cloned human erythropoietin gene in CHO cells

1Kramerova I. A., 1Zelenin M. G., 1Vorontsova M. M., 1Kolobkov S. L., 1Monakova T. E.

Research Institute of Biomedical Technology, Ministry of Public Health of the USSR
Moscow, USSR

Abstract

In the previous study the human gene encoding erythropoietin, the principal factor of erythroid cells differentiation, has been isolated from human genomic phage library. In this paper the plasmid pSVEpo containing erythropoietin gene under the control of promoter of early SV40 genes is obtained. The biologically active erythropoietin has been detected in conditioned media from CHOtk^- cells transfected with pSVEpo plasmid. The plasmid pSVEpo may be used in somatic gene therapy experiments.

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References

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