Білки-партнери субодиниці eEF1Bβ комплексу елонгації трансляції eEF1B в ядерній фракції клітин карциноми легені людини

Капустян, Л. М.; Дадлез, М.; Негруцький, Б. С.

doi:10.7124/bc.00095D

Biopolym. Cell. 2017; 33(4):243-255.

http://dx.doi.org/10.7124/bc.00095D

Структура та функції біополімерів

Білки-партнери субодиниці eEF1Bβ комплексу елонгації трансляції eEF1B в ядерній фракції клітин карциноми легені людини

1Капустян Л. М., 2Дадлез М., 1Негруцький Б. С.

Інститут молекулярної біології і генетики НАН України
Вул. Академіка Заболотного, 150, Київ, Україна, 03680
Інститут біохімії і біофізики Польської академії наук
вул. Павінського, 5a, Варшава, Польща, 02-106

Abstract

Мета. Виявити нових білків-партнерів фактора трансляції eEF1Bβ в ядрі клітин карциноми легені. Методи. Білки-партнери eEF1Bβ, отримані з ядерного екстракту клітин А549 методом ко-імунопреципітації (co-IP), були ідентифіковані за допомогою высокоефективної рідинної хроматографії з тандемною мас-спектрометрією (LC-MS/MS). Подальше підтвердження білків-партнерів проводили із використанням опублікованих даних глобального, кількісного і динамічного картування субклітинної локалізації білків за допомогою програми Mapofthecell. Результати. 104 білки, які взаємодіють із eEF1Bβ в ядерній фракції клітин карциноми легені A549 були ідентифіковані мас-спектрометрією. Проміж цих білків, 9 партнерів eEF1Bβ були підтверджені даними із прецизійного субклітинного фракціонування. За допомогою функціонального аналізу ці білки-партнери можуть бути поділені на про-онкогенну і нейтральну/анти-онкогенну групи. Передбачено, що ці групи можуть бути просторово розділені в ракових клітинах людини. Висновки. Запропоновано, що eEF1Bβ може бути проміжною ланкою між онкогенною і нейтральною/пухлиносупресорною групами партнерів цього білка в ядрі ракових клітин. Розшифрування можливої ролі залучення eEF1Bβ до про-ракової або анти-раковими спільнот білкових партнерів цього білку може бути предметом подальших дослiджень.

Keywords: eEF1Bβ, білок-білкові взаємодії, клітини A549, ILF2, ILF3, HNRNPU, CELF1, DDX6, CCNT1, PA2G4, SPATS2L, USP39

Повний текст: (PDF, англійською)

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