Biopolym. Cell. 2017; 33(4):243-255.
http://dx.doi.org/10.7124/bc.00095D
Structure and Function of Biopolymers
Protein partners of the eEF1Bβ subunit of the translation elongation complex eEF1B in the nuclear fraction of human lung carcinoma cells
1Kapustian L. M., 2Dadlez M., 1Negrutskii B. S.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  2. Institute of Biochemistry and Biophysics, Polish Academy of Sciences
    5a, Pawinskiego, Warsaw, Poland, 02-106

Abstract

Aim. To identify novel protein partners of translation factor eEF1Bβ in nucleus of human lung carcinoma cells. Methods. Protein partners of eEF1Bβ in the nuclear fraction of A549 cells were identified by co-immunoprecipitation (co-IP) combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS). Specific protein partners of eEF1Bβ were further selected by using the results of previously published global, quantitative and dynamic mapping of protein subcellu-lar localization with help of “Mapofthecell” program. Results. 104 high-scored proteins interact-ing with eEF1Bβ in the nuclear fraction of A549 cells have been identified by mass-spectrometry. Among these proteins, 9 partners of eEF1Bβ were confirmed by the co-fractionation approach. Functional analysis of the partners has divided them on the pro-oncogenic (lung-cancer related) and neutral/anti-oncogenic moieties. These two groups are estimated to be spatially separated in human cancer cells. Conclusions. The position of eEF1Bβ as a link between the oncogenic and neutral/tumor-suppressor moieties of its protein partners in nucleus of lung cancer cells is sug-gested. Deciphering of a possible role of the eEF1Bβ distribution between the pro-cancer or anti-cancer communities of its protein partners can be a subject of further research.
Keywords: eEF1Bβ, protein-protein interactions, A549 cells, ILF2, ILF3, HNRNPU, CELF1, DDX6, CCNT1, PA2G4, SPATS2L, USP39
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