Biopolym. Cell. 2013; 29(1):33-41.
http://dx.doi.org/10.7124/bc.000803
Огляди
Нехромосомний цитогенетичний аналіз соматичних клітин ссавців
1Ковальова О. А., 1Бездєнєжних Н. О., 1Кудрявець Ю. Й.
  1. Інститут експериментальної патології, онкології і радіобіології ім. Р. Є. Кавецького
    вул. Васильківська, 45, Київ, Україна, 03022

Abstract

В огляді розглянуто мутаційні події, які відбуваються в соматичних клітинах ссавців під впливом різних ендо- та екзогенних факторів. Нехромосомний метод дослідження дозволяє враховувати сукупність характеристик клітини без трудомісткого аналізу безпосередньо самих хромосом. У результаті можна отримати інформацію про мітотичний (фази мітозу, кількість ядер на клітину, мікроядра, патології мітозу) і вітальний (мітотичний індекс, апоптоз) статуси клітини, а також про стан цілісності хромосом (наявність нуклеоплазматичних мостів, ядерних протрузій, фрагментації хромосом, мікроядер). Залежно від досліджуваного матеріалу (еритроцити і лімфоцити периферійної крові, клітини букального епітелію, постійні клітинні лінії тощо) можна обрати комплекс цитогенетичних характеристик, які є найінформативнішими для кожного окремого випадку при виявленні мутаційних спектрів у соматичних клітинах ссавців.
Keywords: мутагенез, соматична клітина, цитогенетичний аналіз, патологія
Повний текст: (PDF, російською) (PDF, англійською)

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