Biopolym. Cell. 2007; 23(1):21-27.
Молекулярна та клітинна біотехнології
Ендофiтнi бактерiї картоплi in vitro, активованi
екзогенними непатогенними бактерiями
- Інститут агроекології і природокористування НААН України
вул. Метрологічна 12, Київ, Україна, 03680 - Інститут молекулярної біології і генетики НАН України
Вул. Академіка Заболотного, 150, Київ, Україна, 03680 - Інститут мікробіології і вірусології ім. Д. К. Заболотного НАН України
вул. Академіка Заболотного, 154, Київ, Україна, 03680 - Інститут картоплярства УААН
вул. Чкалова, 22, смт. Немiшаєве, Бородянський р-н, Київська обл. Україна, 07853
Abstract
Мiкробiологiчним i незалежним вiд культивування бактерiй методами показано присутнiсть як культивованих, так i некультивованих форм ендофiтних бактерiй у рослинах - регенерантах картоплi, якi вирощують за умов in vitro. Введення ризобактерiй Pseudomonas fluorescens IМБГ163 у рослини картоплi паралельно з рослинним матерiалом у культуру in vitro збiльшувало кiлькiсть видiв ендофiтних бактерiй корiння першого вегетативного поколiння рослин, але при подальшому клональному мiкророзмноженнi рослин кiлькiсть виявлених ендофiтiв зменшувалася. Ендофiтнi iзоляти сортiв картоплi Загадка та Нiгру, активованi ризобактерiєю, виявляли кориснi для рослин властивостi.
Keywords: ендофiтнi бактерiї, картопля in vitro, бiотичний стресор, Pseudomonas fluorescens IМБГ163
Повний текст: (PDF, українською) (PDF, англійською)
References
[1]
Rosenblueth M, Mart?nez-Romero E. Bacterial endophytes and their interactions with hosts. Mol Plant Microbe Interact. 2006;19(8):827-37.
[2]
Kozyrovska NO. Molecular and genetic aspects of external and internal colonization plants by beneficial bacteria. Biopolym Cell. 2001; 17(1):20-8.
[3]
Siciliano SD, Fortin N, Mihoc A, Wisse G, Labelle S, Beaumier D, Ouellette D, Roy R, Whyte LG, Banks MK, Schwab P, Lee K, Greer CW. Selection of specific endophytic bacterial genotypes by plants in response to soil contamination. Appl Environ Microbiol. 2001;67(6):2469-75.
[4]
Ara?jo WL, Marcon J, Maccheroni W Jr, Van Elsas JD, Van Vuurde JW, Azevedo JL. Diversity of endophytic bacterial populations and their interaction with Xylella fastidiosa in citrus plants. Appl Environ Microbiol. 2002;68(10):4906-14.
[5]
Pirttil? AM, Pospiech H, Laukkanen H, Myllyl? R, Hohtola A. Seasonal variations in location and population structure of endophytes in buds of Scots pine. Tree Physiol. 2005;25(3):289-97.
[6]
Sorochinskiy BV, Kozyrovska NO. Biotechnological aspects of phytoremediation of the environment of radionuclide contamination. Agrobiotekhnologiya. 1998; 2: 123-30.
[7]
Verma SC, Ladha JK, Tripathi AK. Evaluation of plant growth promoting and colonization ability of endophytic diazotrophs from deep water rice. J Biotechnol. 2001;91(2-3):127-41.
[8]
Caballero-Mellado J, Mart?nez-Aguilar L, Paredes-Valdez G, Santos PE. Burkholderia unamae sp. nov., an N2-fixing rhizospheric and endophytic species. Int J Syst Evol Microbiol. 2004;54(Pt 4):1165-72.
[9]
Normander B, Prosser JI. Bacterial origin and community composition in the barley phytosphere as a function of habitat and presowing conditions. Appl Environ Microbiol. 2000;66(10):4372-7.
[10]
Krechel A, Faupel A, Hallmann J, Ulrich A, Berg G. Potato-associated bacteria and their antagonistic potential towards plant-pathogenic fungi and the plant-parasitic nematode Meloidogyne incognita (Kofoid & White) Chitwood. Can J Microbiol. 2002;48(9):772-86.
[11]
Sessitsch A, Reiter B, Berg G. Endophytic bacterial communities of field-grown potato plants and their plant-growth-promoting and antagonistic abilities. Can J Microbiol. 2004;50(4):239-49.
[12]
Garbeva P, Overbeek LS, Vuurde JW, Elsas JD. Analysis of Endophytic Bacterial Communities of Potato by Plating and Denaturing Gradient Gel Electrophoresis (DGGE) of 16S rDNA Based PCR Fragments. Microb Ecol. 2001;41(4):369-383.
[13]
Amann RI, Ludwig W, Schleifer KH. Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol Rev. 1995;59(1):143-69.
[14]
Reiter B, Pfeifer U, Schwab H, Sessitsch A. Response of endophytic bacterial communities in potato plants to infection with Erwinia carotovora subsp. atroseptica. Appl Environ Microbiol. 2002;68(5):2261–8.
[15]
Kovalchuk MV, Lytvynenko TL, Kononuchenko OV, Voznyuk TM, Rymar SYu, Negrutska VV, Kozyrovska NO. Colonization capacity and monitoring of the biocontrol agent Pseudomonas sp. IMBG163 on wheat roots. Biopolym Cell. 2004; 20(6):530-4.
[16]
King EO, Ward MK, Raney DE. Two simple media for the demonstration of pyocyanin and fluorescin. J Lab Clin Med. 1954;44(2):301-7.
[17]
Podolich OV, Kovalchuk MV, Lytvynenko TL, Kononuchenko OV, Kozyrovska NO, Petyukh GP. The features of in vitro potato plants colonization by bacteria Pseudomonas sp. IMBG163. Agroekologichnyy Zhurnal. 2005; 2: 61-4.
[18]
Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue culture. Proc Int Citrus Symp. 1969; 3: 1155-61.
[19]
Romanovskaia VA, Stoliar SM, Malashenko IuR, Shatokhina ES. [The effect of long-acting radiation on the diversity of heterotrophic bacteria in the soils of a 10-kilometer area around the Chernobyl Atomic Electric Power Station]. Mikrobiol Z. 1996;58(5):3-12.
[20]
Starr MP, Chatterjee AK, Starr PB, Buhanan GE. Enzymatic degradation of polygalacturonic acid by Yersinia and Klebsiella species in relation to clinical laboratory procedures. J Clin Microbiol. 1977; 6: 379-86.
[21]
Miller JH. Experiments in molecular genetics. Cold Spring Harbor Laboratory, 1972, 466 p.
[22]
Wood PJ. The use of dye-polysaccharide interactions in β-D-glucanase assay. Carbohydr Res. 1981; 94(2):19-23.
[23]
Tang YW, Bonner J. The enzymatic inactivation of indoleacetic acid; some characteristics of the enzyme contained in pea seedlings. Arch Biochem. 1947;13(1):11-25.
[24]
Kovalchuk MV, Negrutska VV, Kozyrovska NO. Features of some pseudomonas as candidates for creation a new drugs class by the"Dual" technology. Agroekologichnyy Zhurnal. 2004; 2: 41-45.
[25]
Sambrook J, Fritisch EF, Maniatis T. Molecular cloning. A laboratory manual. New York: Cold Spring Harbor Lab. press, 1989. 1962 p.
[26]
Liu WT, Marsh TL, Cheng H, Forney LJ. Characterization of microbial diversity by determining terminal restriction fragment length polymorphisms of genes encoding 16S rRNA. Appl Environ Microbiol. 1997;63(11):4516-22.
[27]
Wilson M, Hirano SS, Lindow SE. Location and survival of leaf-associated bacteria in relation to pathogenicity and potential for growth within the leaf . Appl Environ Microbiol. 1999;65(4):1435-43.
[28]
Doronina NV, Ivanova EG, Suzina NF, Trotsenko IuA. [Methanotrophs and methylobacteria are found in woody plant tissues within a winter period]. Mikrobiologiia. 2004;73(6):817-24.
[29]
Zarnowski R, Felske A, Ellis RJ, Geuns JM, Pietr SJ. A Methylobacterium-like organism from algal crusts covering silicone rubber electric insulators in Africa. J Appl Microbiol. 2002;93(6):1012-9.
[30]
Idris R, Trifonova R, Puschenreiter M, Wenzel WW, Sessitsch A. Bacterial communities associated with flowering plants of the Ni hyperaccumulator Thlaspi goesingense. Appl Environ Microbiol. 2004;70(5):2667-77.
[31]
Okubara PA, Paulitz TC. Root defense responses to fungal pathogens: a molecular perspective. Plant and Soil. 2005; 274(1-2): 215-26.
[32]
Podolich OV, Litvinenko TL, Voznyuk TM, Kovalchuk MV, Kozyrovska NO. Identifying groups endophitous bacteria in aseptic potato plants after inoculation Pseudomonas sp. IMBG163. Nauk Visn Uzhgorod Univ. 2006; (18):165-70.