Biopolym. Cell. 2006; 22(5):375-383.
http://dx.doi.org/10.7124/bc.000745
Молекулярна біофізика
Вплив хлороформу на стан води, адсорбованої сироватковим альбуміном
1Нечипор О. В., 1Гунько В. М., 1Барвінченко В. М., 1Туров В. В.
  1. Інститут хімії поверхні НАН України
    вул. Генерала Наумова, 17, Київ, Україна, 03164

Abstract

Методом 1Н ЯМР спектроскопії з виморожуванням рідкої фази вивчено будову гідратної оболонки ліофілізованого і сорбованого на поверхні високодисперсного кремнезему сироват­кового альбуміну людини, що знаходиться в слабополярному розчиннику (хлороформі) або при його додаванні до гідратованої системи. Показано, що вплив хлороформу може спри­чинювати зміну об'ємів внутрішніх областей білкової глобули, які характеризуються різною гідрофільністю і заповнених структурованою водою, що не замерзає за температури Т < 273 К.
Keywords: сироватковий альбумін людини, високо­ дисперсний кремнезем, хлороформ, 1Н ЯМР спектроскопія
Повний текст: (PDF, російською) (PDF, англійською)

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