Biopolym. Cell. 2006; 22(5):375-383.
http://dx.doi.org/10.7124/bc.000745
Molecular Biophysics
The influence of weakly polar solvent chloroform on the state of water adsorbed by serum albumin
1Nechypor O. V., 1Gun'ko V. M., 1Barvinchenko V. M., 1Turov V. V.
  1. Institute of Surface Chemistry, NAS of Ukraine
    17, Generala Naumova Str., Kyiv, Ukraine, 03164

Abstract

The hydrate shell structure of dry human serum albumin and albumin adsorbed on a surface of highly disperse silica in a weakly polar solvent (chloroform) or with addition of this solvent was studied by 1H NMR spectroscopy with layer-by-layer freezing out of a liquid phase. The influence of chloroform can result in changes in the ratio of volumes of internal regions of protein globule characterized by different hydration level and filled up with structured water unfrozen at T < 273K.
Keywords: human serum albumin, highly disperse silica, chloroform, 1H NMR spectroscopy
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