Biopolym. Cell. 1999; 15(1):67-74.
http://dx.doi.org/10.7124/bc.000508
Віруси та клітина
Нові пакувальні клітини на основі адаптованого варіанта вірусу саркоми Рауса
1Кайда І. В., 2Серое С. М., 1Михайлик А. А., 1Болтовець П. М., 1Кашуба В. І., 1Риндич А. В.
  1. Інститут молекулярної біології і генетики НАН України
    Вул. Академіка Заболотного, 150, Київ, Україна, 03680
  2. Інститут молекулярної біології ім. В. А. Енгельгардта РАН
    вул. Вавилова, 32, Москва, Російська Федерація, 119991

Abstract

Створено нові пакувальні клітини F18/pJD, які містять вірус-помічник на основі трансформаційно-дефектного мутан­та адаптованого до качок варіанту празького штама вірусу саркоми Рауса (td daPr-RSV-C), що продукують реплікативнодефектний ретровірусний вектор з титром 106 КУО/мл. Дилетування цис-активних послідовностей V пакувальної області та. обернених повторів (Е), фланкуючих src, адаптова­ного варіанту ретровірусу при створенні конструкції вірусу-помічника дозволим) отримати пул векторного вірусу без послідовностей вірусу-помічника.
Повний текст: (PDF, російською)

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