Нові пакувальні клітини на основі адаптованого варіанта вірусу саркоми Рауса

Кайда, І. В.; Серое, С. М.; Михайлик, А. А.; Болтовець, П. М.; Кашуба, В. І.; Риндич, А. В.

doi:10.7124/bc.000508

Biopolym. Cell. 1999; 15(1):67-74.

http://dx.doi.org/10.7124/bc.000508

Віруси та клітина

Нові пакувальні клітини на основі адаптованого варіанта вірусу саркоми Рауса

1Кайда І. В., 2Серое С. М., 1Михайлик А. А., 1Болтовець П. М., 1Кашуба В. І., 1Риндич А. В.

Інститут молекулярної біології і генетики НАН України
Вул. Академіка Заболотного, 150, Київ, Україна, 03680
Інститут молекулярної біології ім. В. А. Енгельгардта РАН
вул. Вавилова, 32, Москва, Російська Федерація, 119991

Abstract

Створено нові пакувальні клітини F18/pJD, які містять вірус-помічник на основі трансформаційно-дефектного мутанта адаптованого до качок варіанту празького штама вірусу саркоми Рауса (td daPr-RSV-C), що продукують реплікативнодефектний ретровірусний вектор з титром 10⁶ КУО/мл. Дилетування цис-активних послідовностей V пакувальної області та. обернених повторів (Е), фланкуючих src, адаптованого варіанту ретровірусу при створенні конструкції вірусу-помічника дозволим) отримати пул векторного вірусу без послідовностей вірусу-помічника.

Повний текст: (PDF, російською)

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