Biopolym. Cell. 1995; 11(3-4):82-87.
Отримання і молекулярно-біологічний аналіз трансгенних
рослин гороху (Pisum sativum L.), які містять
Ds-елемент кукурудзи
- Інститут клітинної біології та генетичної інженерії НАН України
вул. Академіка Заболотного, 148, Київ, Україна, 03680
Abstract
Рослини гороху (P. sativum L.) з підвищеною регенераційною здатністю трансформували конструкцією, яка містить Ds-елемет системи контролюючих елементів Ac/Ds кукурудзи. Трансформацію здійснювали кокультивуванням рослинних тканин з Agrobacterium tumefaciens. Селекційовано ряд стійких до канаміцинсульфату ліній. За допомогою методів ПЦР-аналізу і Саузерн-блотинг-гібридизації показано інтеграцію Ds-елемента до складу геномної ДНК гороху.
Повний текст: (PDF, російською)
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