Biopolym. Cell. 1995; 11(3-4):82-87.
http://dx.doi.org/10.7124/bc.0003F0
Obtaining and molecular biological analysis transgenic pea plants, contained the maize Ds-element
1Rachek L. I., 1Storozhenko S. V., 1Kuchuk N. V.
  1. Institute of Cell Biology and Genetic Engineering, NAS of Ukraine
    148, Akademika Zabolotnogo Str., Kyiv, Ukraine, 03680

Abstract

Pea plants have been transformed with the gene construction containing Ds-element of Ac/Ds maize transposon system. The construction was introduced into pea plants using Agrobaclerium tumefaciens-mediaied transformation. Several transgenic plants have been selected on the nutritional medium contained the kanamycinsulphate as a selective agent. Concentration of NPTII protein was determined by ELISA assay. Using PCR-analyses and Southern blotting hybridization an integration of Ds-element in plant genome has been confirmed.
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References

[1] Gerats AGM, Beld M, Huits H, Prescott A. Gene tagging inPetunia hybrida using homologous and heterologous transposable elements. Dev Genet. 1989;10(6):561–8.
[2] Ellis JG, Finnegan EJ, Lawrence GJ. Developing a transposon tagging system to isolate rust-resistance genes from flax. Theor Appl Genet. 1992;85(1):46-54.
[3] Haring MA, Rommens CM, Nijkamp HJ, Hille J. The use of transgenic plants to understand transposition mechanisms and to develop transposon tagging strategies. Plant Mol Biol. 1991;16(3):449-61.
[4] Shepherd NS, Rhoades MM, Dempsey E. Genetic and molecular characterization of a-mrh-Mrh, a new mutable system of Zea mays. Dev Genet. 1989;10(6):507-19.
[5] Coen ES, Carpenter R. A semi-dominant allele, niv-525, acts in trans to inhibit expression of its wild-type homologue in Antirrhinum majus. EMBO J. 1988;7(4):877-83.
[6] Grandbastien MA, Spielmann A, Caboche M. Tnt1, a mobile retroviral-like transposable element of tobacco isolated by plant cell genetics. Nature. 1989;337(6205):376-80.
[7] Tsay YF, Frank MJ, Page T, Dean C, Crawford NM. Identification of a mobile endogenous transposon in Arabidopsis thaliana. Science. 1993;260(5106):342-4.
[8] Baker B, Schell J, Lörz H, Fedoroff N. Transposition of the maize controlling element "Activator" in tobacco. Proc Natl Acad Sci U S A. 1986;83(13):4844-8.
[9] Belzile F, Lassner MW, Tong Y, Khush R, Yoder JI. Sexual transmission of transposed activator elements in transgenic tomatoes. Genetics. 1989;123(1):181-9.
[10] Schmidt R, Willmitzer L. The maize autonomous element Activator (Ac) shows a minimal germinal excision frequency of 0.2%-0.5% in transgenic Arabidopsis thaliana plants. Mol Gen Genet. 1989;220(12):11-24.
[11] Van Sluys MA, Tempé J, Fedoroff N. Studies on the introduction and mobility of the maize Activator element in Arabidopsis thaliana and Daucus carota. EMBO J. 1987;6(13):3881-9.
[12] Izawa T, Miyazaki C, Yamamoto M, Terada R, Iida S, Shimamoto K. Introduction and transposition of the maize transposable element Ac in rice (Oryza sativa L.). Mol Gen Genet. 1991;227(3):391-6.
[13] Sugimoto K, Otsuki Y, Saji S, Hirochika H. Transposition of the maize Ds element from a viral vector to the rice genome. Plant J. 1994;5(6):863-71.
[14] Houba-Hérin N, Domin M, Pédron J. Transposition of a Ds element from a plasmid into the plant genome in Nicotiana plumbaginifolia protoplast-derived cells. Plant J. 1994;6(1):55-66.
[15] Houba-Hérin N, Becker D, Post A, Larondelle Y, Starlinger P. Excision of a Ds-like maize transposable element (Ac delta) in a transient assay in Petunia is enhanced by a truncated coding region of the transposable element Ac. Mol Gen Genet. 1990;224(1):17-23.
[16] Chuck G, Robbins T, Nijjar C, Ralston E, Courtney-Gutterson N, Dooner HK. Tagging and Cloning of a Petunia Flower Color Gene with the Maize Transposable Element Activator. Plant Cell. 1993;5(4):371-378.
[17] Bancroft I, Jones JD, Dean C. Heterologous transposon tagging of the DRL1 locus in Arabidopsis. Plant Cell. 1993;5(6):631-8.
[18] Aarts MG, Dirkse WG, Stiekema WJ, Pereira A. Transposon tagging of a male sterility gene in Arabidopsis. Nature. 1993;363(6431):715-7.
[19] Birnboim HC, Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979;7(6):1513-23.
[20] Hoekema A, Hirsch PR, Hooykaas PJJ, Schilperoort RA. A binary plant vector strategy based on separation of vir- and T-region of the Agrobacterium tumefaciens Ti-plasmid. Nature. 1983;303(5913):179–80.
[21] Ditta G, Stanfield S, Corbin D, Helinski DR. Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti. Proc Natl Acad Sci U S A. 1980;77(12):7347-51.
[22] Zubko EI, Kuchuk NV, Tumanova LG, Vikonskaya NA, Gleba YuYu. Genetic transformation of pea plants mediated by Agrobacterium tumefaciens. Biopolym Cell. 1990; 6(3):77-80.
[23] Gamborg OL, Miller RA, Ojima K. Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res. 1968;50(1):151-8.
[24] Deak M, Kiss GB, Koncz C, Dudits D. Transformation of Medicago by Agrobacterium mediated gene transfer. Plant Cell Rep. 1986;5(2):97-100.
[25] Storozhenko SV, Kravets OA, Pogrebnyak NYa, Gleba YuYu. Isolation of the transgenic potato plants expressing the mutant gene coding for acetolactate synthase from Arabidopsis thaliana. Biopolym Cell. 1994; 10(1):67-71.
[26] Murray MG, Thompson WF. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res. 1980;8(19):4321-5.
[27] Nagel RJ, Manners JM, Birch RG. Evaluation of an ELISA assay for rapid detection and quantification of neomycin phosphotransferase II in transgenic plants. Plant Mol Biol Rep. 1992;10(3):263–72.
[28] Church GM, Gilbert W. Genomic sequencing. Proc Natl Acad Sci U S A. 1984;81(7):1991-5.
[29] Feinberg AP, Vogelstein B. "A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum. Anal Biochem. 1984;137(1):266-7.