Biopolym. Cell. 1986; 2(2):69-73.
Structure and Function of Biopolymers
Catalytic activity of aspartate aminotransferase microcrystals
1Kochkina V. M.
  1. Institute of Molecular Biology, Academy of Sciences of the USSR
    Moscow, USSR


The enzymic activity of crystalline cytosolic aspartate aminotransferase (L-aspartate: aminotransferase 2-oxoglutarate, EC was determined in suspensions of micro-crystals in 50 % ammonium sulphate and 20 % (wt/vol) polyethylene glycol. The crystals (1-2 μm wide) were small enough to preclude diffusional rate limitation. The catalytic activity of the crystalline enzyme is 32 % and 40 % of the enzyme activity in solution, respectively.


[1] Doscher MS, Richards FM. The activity of an enzyme in the crystalline state: ribonuclease S. J Biol Chem. 1963; 238(7):2399-2406.
[2] Spilburg CA, Bethune JL, Valee BL. Kinetic properties of crystalline enzymes. Carboxypeptidase A. Biochemistry. 1977;16(6):1142-50.
[3] Alter GM, Leussing DL, Neurath H, Vallee BL. Kinetic properties of carboxypeptidase B in solutions and crystals. Biochemistry. 1977;16(16):3663-8.
[4] Kirsten H, Christen P. Catalytic activity of non-cross-linked microcrystals of aspartate aminotransferase in poly(ethylene glycol). Biochem J. 1983;211(2):427-34.
[5] Malashkevich VN, Kochkina VM, Torchinskii IuM, Arutiunian EG. Conformational changes in cytosol aspartate aminotransferase induced by oxoglutarate. Dokl Akad Nauk SSSR. 1982;267(5):1257-61.
[6] Borisov VV, Borisova SN, Sosfenov NI, Dikson KhB. Conformation of aspartate aminotransferase in crystals Mol Biol (Mosk). 1983;17(4):705-13.
[7] Jenkins W. T., Yphaniis D. A., Sizer . . W. Glutamic aspartic transaminase. J. Biol. Chem, 1959. 234, N 1:51-57.
[8] Bertland LH, Kaplan NO. Studies on the conformations of the multiple forms of chicken heart aspartate aminotransferase. Biochemistry. 1970;9(13):2653-65.
[9] Kochkina VM. Influence of metal ions on the crystal size aspartate transaminase, getting tyazheloatomnyh derived enzyme crystals. Kristallografiya. 1983; 28(5):1013-7.
[10] Kiick DM, Cook PF. H studies toward the elucidation of the auxiliary catalyst for pig heart aspartate aminotransferase. Biochemistry. 1983;22(2):375-82.