Biopolym. Cell. 1989; 5(3):39-43.
http://dx.doi.org/10.7124/bc.0000B5
Структура та функції біополімерів
Вплив іонної сили розчину на енергію надспіралізації ДНК
1Шурдов М. А., 1Груздев О. Д.
  1. Інститут цитології і генетики, Сибірського відділення АН СРСР
    Новосибірськ, СРСР

Abstract

Визначено залежність вільної енергії надспіралізації g > Kσ2 від концентрації NaCl у розчині за вимірюваннями ізотерм зв’язування бромистого етидію з ДНК плазміди pBR322. Показано, що зі зменшенням концентрації солі від 0,3 до 0,01 M значення константи К зростає більш ніж удвічі, а кількість надвитків помітно падає. Енергія надспіралізації ДНК зменшується приблизно на 20 %.
Повний текст: (PDF, російською)

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