Biopolym. Cell. 2023; 39(4):283-298.
Молекулярна та клітинна біотехнології
Комплексне дослідження гормоннезалежного високопродуктивного штаму культури тканин Rauvolfia serpentina — джерела індольних алкалоїдів
- Інститут молекулярної біології і генетики НАН України
Вул. Академіка Заболотного, 150, Київ, Україна, 03143 - Фірма “Otava”
Вул. Академіка Заболотного, 150, Київ, Україна, 03143
Abstract
Мета. Характеристика нового гормононезалежного штаму К-27М культури тканин R. serpentina. Методи. Культура тканин, біохімічний та цитологічний аналіз, методи статистики. Результати. Максимальний вихід біомаси був на 69-й день росту, кількість біомаси зростала за пасаж у 15–18 разів. Культура характеризувалася значним поліморфізмом клітин та ядер, протягом пасажу їх розмір змінювався, що віддзеркалювало фази росту культури. Штам К-27М R. serpentina (2n=22) є гетерогенною міксоплоїдною клітинною популяцією. Модальний клас формували клітини з числом хромосом від 22 до 33 (понад 65% клітин, що ділилися). Припускається зв’язок рівня плоїдності культивованих клітин з накопиченням алкалоїдів. Максимальне накопичення індольних алкалоїдів виявлено на 88–108-й день росту культури. Висновки. Штам К-27М R. serpentina, вирощений на простому за складом живильному середовищі, є перспективним продуцентом індольних алкалоїдів. За одну добу з 1 л середовища вихід становить: 540 мг сухої біомаси, що містить 4% індольних алкалоїдів, у т.ч. 0,79% аймаліну, або суми індольних алкалоїдів – 21,5 мг; аймаліну – 4,3 мг.
Keywords: культура тканин рослин, Rauvolfia serpentina, індольні алкалоїди, клітинна селекція, , , ,
Повний текст: (PDF, англійською)
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