Biopolym. Cell. 2023; 39(2):77-89.
Огляди
Особливості ПЛР діагностики сибірки
- Інститут молекулярної біології і генетики НАН України
Вул. Академіка Заболотного, 150, Київ, Україна, 03143
Abstract
Молекулярні методи діагностики за певних умов мають значну перевагу над тривалими класичними мікробіологічними методами. Через високопатогенні властивості Bacillus anthracis необхідно впровадити у практику швидкі методи ідентифікації збудника у разі виникнення біологічної загрози розповсюдження збудника інфекції серед сприятливих тварин, людей та зараження території. Ідентифікація B. anthracis є складною через спорову та вегетативну форму існування та схожість із близькоспорідненими видами. Україна є неблагополучною державою щодо сибірки. Впровадження надійних, чутливих і специфічних молекулярних методів діагностики є пріоритетним напрямком у вирішенні питання біобезпеки.
Keywords: Bacillus anthracis, діагностика, ідентифікація, експрес–тести, мікробіологічні дослідження, методи молекулярної мікробіології
Повний текст: (PDF, англійською)
References
[1]
Doganay M, Demiraslan H. Human anthrax as a re-emerging disease. Recent Pat Antiinfect Drug Discov. 2015; 10(1):10-29.
[3]
Sinitsyn VA, Yanenko UM, Zaviryukha GA, Vasileva TB, Tarasov OA, Kosyanchuk NI, Mizukina LM. The situation of anthrax which is on the territory of Ukraine. Ukrainian Jornal of Ecology. 2019; 9(3):113-7.
[4]
Anthrax in Humans and Animals. 4th ed. Geneva: World Health Organization; 2008.
[5]
Hossein A, Hossein H, Ebrahim M. Cloning, fusion, and expression of domain a-1 protective antigen (PA20) of Bacillus anthracis and N-terminal ipaD gene of Shigella in E. coli. Qom Univ Med Sci J. 2015; 9:20-9.
[6]
Freidlander AM. Anthrax. In FR Sidell, ET Takafuji, and DR Franz (eds.), Medical Aspects of Chemical and Biological Warfare. Office of the Surgeon General, Washington, DC. 1997; 467-78.
[7]
Hoffmaster AR, Fitzgerald CC, Ribot E, Mayer LW, Popovic T. Molecular subtyping of Bacillus anthracis and the 2001 bioterrorism-associated anthrax outbreak, United States. Emerg Infect Dis. 2002; 8(10):1111-6.
[8]
Bengis RG, Frean J. Anthrax as an example of the One Health concept. Rev Sci Tech. 2014; 33(2):593-604.
[9]
Baillie LW. Bacillus anthracis, a story of nature subverted by man. Lett Appl Microbiol. 2005; 41(3):227-9.
[10]
Kamal SM, Rashid AK, Bakar MA, Ahad MA. Anthrax: an update. Asian Pac J Trop Biomed. 2011; 1(6):496-501.
[11]
Carlson CJ, Kracalik IT, Ross N, Alexander KA, Hugh-Jones ME, Fegan M, Elkin BT, Epp T, Shury TK, Zhang W, Bagirova M, Getz WM, Blackburn JK. The global distribution of Bacillus anthracis and associated anthrax risk to humans, livestock and wildlife. Nat Microbiol. 2019; 4(8):1337-43.
[13]
Owen JL, Yang T, Mohamadzadeh M. New insights into gastrointestinal anthrax infection. Trends Mol Med. 2015; 21(3):154-63.
[14]
Swartz MN. Recognition and management of anthrax--an update. N Engl J Med. 2001; 345(22):1621-6.
[15]
Keim P, Smith KL. Bacillus anthracis evolution and epidemiology. Curr Top Microbiol Immunol. 2002; 271:21-32.
[16]
Keim P, Van Ert MN, Pearson T, Vogler AJ, Huynh LY, Wagner DM. Anthrax molecular epidemiology and forensics: using the appropriate marker for different evolutionary scales. Infect Genet Evol. 2004; 4(3):205-13.
[17]
Hugh-Jones M, Blackburn J. The ecology of Bacillus anthracis. Mol Aspects Med. 2009; 30(6):356-67.
[18]
Riojas MA, Kiss K, McKee ML, Hazbón MH. Multiplex PCR for species-level identification of Bacillus anthracis and detection of pXO1, pXO2, and related plasmids. Health Secur. 2015; 13(2):122-9.
[19]
Okinaka RT, Cloud K, Hampton O, Hoffmaster AR, Hill KK, Keim P, Koehler TM, Lamke G, Kumano S, Mahillon J, Manter D, Martinez Y, Ricke D, Svensson R, Jackson PJ. Sequence and organization of pXO1, the large Bacillus anthracis plasmid harboring the anthrax toxin genes. J Bacteriol. 1999; 181(20):6509-15.
[20]
Liang X, Zhu J, Zhao Z, Zheng F, Zhang H, Wei J, Ji Y, Ji Y. The pag Gene of pXO1 Is Involved in Capsule Biosynthesis of Bacillus anthracis Pasteur II Strain. Front Cell Infect Microbiol. 2017; 7:203.
[22]
Vilas-Bôas GT, Peruca AP, Arantes OM. Biology and taxonomy of Bacillus cereus, Bacillus anthracis, and Bacillus thuringiensis. Can J Microbiol. 2007; 53(6):673-87.
[23]
Friebe S, van der Goot FG, Bürgi J. The Ins and Outs of Anthrax Toxin. Toxins (Basel). 2016; 8(3):69.
[24]
Bann JG. Anthrax toxin protective antigen--insights into molecular switching from prepore to pore. Protein Sci. 2012; 21(1):1-12.
[25]
Pilo P, Frey J. Bacillus anthracis: molecular taxonomy, population genetics, phylogeny and patho-evolution. Infect Genet Evol. 2011; 11(6):1218-24.
[26]
Abshire TG, Brown JE, Ezzell JW. Production and validation of the use of gamma phage for identification of Bacillus anthracis. J Clin Microbiol. 2005; 43(9):4780-8.
[27]
Lechner S, Mayr R, Francis KP, Prüss BM, Kaplan T, Wiessner-Gunkel E, Stewart GS, Scherer S. Bacillus weihenstephanensis sp. nov. is a new psychrotolerant species of the Bacillus cereus group. Int J Syst Bacteriol. 1998; 48(4):1373-82.
[28]
Léonard C, Chen Y, Mahillon J. Diversity and differential distribution of IS231, IS232 and IS240 among Bacillus cereus, Bacillus thuringiensis and Bacillus mycoides. Microbiology (Reading). 1997; 143(8):2537-47.
[29]
Battisti L, Green BD, Thorne CB. Mating system for transfer of plasmids among Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis. J Bacteriol. 1985; 162(2):543-50.
[30]
Hoffmaster AR, Ravel J, Rasko DA, Chapman GD, Chute MD, Marston CK, De BK, Sacchi CT, Fitzgerald C, Mayer LW, Maiden MC, Priest FG, Barker M, Jiang L, Cer RZ, Rilstone J, Peterson SN, Weyant RS, Galloway DR, Read TD, Popovic T, Fraser CM. Identification of anthrax toxin genes in a Bacillus cereus associated with an illness resembling inhalation anthrax. Proc Natl Acad Sci U S A. 2004; 101(22):8449-54.
[31]
Hoffmaster AR, Hill KK, Gee JE, Marston CK, De BK, Popovic T, Sue D, Wilkins PP, Avashia SB, Drumgoole R, Helma CH, Ticknor LO, Okinaka RT, Jackson PJ. Characterization of Bacillus cereus isolates associated with fatal pneumonias: strains are closely related to Bacillus anthracis and harbor B. anthracis virulence genes. J Clin Microbiol. 2006; 44(9):3352-60.
[32]
Ågren J, Hamidjaja RA, Hansen T, Ruuls R, Thierry S, Vigre H, Janse I, Sundström A, Segerman B, Koene M, Löfström C, Van Rotterdam B, Derzelle S. In silico and in vitro evaluation of PCR-based assays for the detection of Bacillus anthracis chromosomal signature sequences. Virulence. 2013; 4(8):671-85.
[33]
Pannucci J, Okinaka RT, Sabin R, Kuske CR. Bacillus anthracis pXO1 plasmid sequence conservation among closely related bacterial species. J Bacteriol. 2002; 184(1):134-41.
[34]
Jernigan JA, Stephens DS, Ashford DA, Omenaca C, Topiel MS, Galbraith M, Tapper M, Fisk TL, Zaki S, Popovic T, Meyer RF, Quinn CP, Harper SA, Fridkin SK, Sejvar JJ, Shepard CW, McConnell M, Guarner J, Shieh WJ, Malecki JM, Gerberding JL, Hughes JM, Perkins BA. Anthrax Bioterrorism Investigation Team. Bioterrorism-related inhalational anthrax: the first 10 cases reported in the United States. Emerg Infect Dis. 2001; 7(6):933-44.
[35]
Scarff JM, Raynor MJ, Seldina YI, Ventura CL, Koehler TM, O'Brien AD. The roles of AtxA orthologs in virulence of anthrax-like Bacillus cereus G9241. Mol Microbiol. 2016; 102(4):545-61.
[36]
Keim P, Gruendike JM, Klevytska AM, Schupp JM, Challacombe J, Okinaka R. The genome and variation of Bacillus anthracis. Mol Aspects Med. 2009; 30(6):397-405.
[37]
Klee SR, Brzuszkiewicz EB, Nattermann H, Brüggemann H, Dupke S, Wollherr A, Franz T, Pauli G, Appel B, Liebl W, Couacy-Hymann E, Boesch C, Meyer FD, Leendertz FH, Ellerbrok H, Gottschalk G, Grunow R, Liesegang H. The genome of a Bacillus isolate causing anthrax in chimpanzees combines chromosomal properties of B. cereus with B. anthracis virulence plasmids. PLoS One. 2010; 5(7):e10986.
[38]
Instructions for laboratory diagnostics of anthrax in humans, in raw materials of animal origin and environmental objects: Order of the Ministry of Health of Ukraine of 21.08.2002, No. 321 [Instrukcii' z laboratornoi' diagnostyky sybirky u ljudej, v syrovyni tvarynnogo pohodzhennja ta ob'jektah dovkillja: Nakaz MOZ Ukrai'ny vid 21.08.2002, N 321]. Available at: https://zakon.rada.gov.ua/rada/show/v0321282-02#Text
[39]
Skrypnyk VG, Rublenko IO, Garkavenko TO, Golovko AM, Zagrebelnyi VO, Ushkalov VO, Deriabin OM, Machuskyi OV, Skrypnyk AV, Pinchuk NG. Laboratory diagnostics of animal anthrax, indication of pathogen and biological agent, raw material of animal origin and environmental objects: scientific and methodological recommendations for ensuring the practical and independent work of specialists of laboratories and research institutions of veterinary medicine, teachers and students [Laboratorna diagnostyka sybirky tvaryn, indykacija zbudnyka z patologichnogo ta biologichnogo materialu, syrovyny tvarynnogo pohodzhennja ta ob'jektiv navkolyshn'ogo seredovyshha: naukovo-metodychni rekomendacii' dlja zabezpechennja praktychnoi' ta samostijnoi' roboty fahivciv laboratorij ta naukovo-doslidnyh ustanov veterynarnoi' medycyny, vykladachiv ta studentiv fakul'tetiv veterynarnoi' medycyny VNZ] - Kyiv, 2014. - 77 p. Available at: https://rep.btsau.edu.ua/handle/BNAU/3416
[40]
Reitsma JB, Rutjes AW, Khan KS, Coomarasamy A, Bossuyt PM. A review of solutions for diagnostic accuracy studies with an imperfect or missing reference standard. J Clin Epidemiol. 2009; 62(8):797-806.
[41]
Beyer W, Glöckner P, Otto J, Böhm R. A nested PCR method for the detection of Bacillus anthracis in environmental samples collected from former tannery sites. Microbiol Res. 1995; 150(2):179-86.
[42]
Ramisse V, Patra G, Garrigue H, Guesdon JL, Mock M. Identification and characterization of Bacillus anthracis by multiplex PCR analysis of sequences on plasmids pXO1 and pXO2 and chromosomal DNA. FEMS Microbiol Lett. 1996; 145(1):9-16.
[43]
Sjöstedt A, Eriksson U, Berglund L, Tärnvik A. Detection of Francisella tularensis in ulcers of patients with tularemia by PCR. J Clin Microbiol. 1997; 35(5):1045-8.
[44]
Jackson PJ, Hugh-Jones ME, Adair DM, Green G, Hill KK, Kuske CR, Grinberg LM, Abramova FA, Keim P. PCR analysis of tissue samples from the 1979 Sverdlovsk anthrax victims: the presence of multiple Bacillus anthracis strains in different victims. Proc Natl Acad Sci U S A. 1998; 95(3):1224-9.
[45]
Turnbull PCB. Guidelines for the Surveillance and Control of Anthrax in Human and Animals. 3rd ed. World Health Organization, 1998.
[46]
Easterday WR, Van Ert MN, Simonson TS, Wagner DM, Kenefic LJ, Allender CJ, Keim P. Use of single nucleotide polymorphisms in the plcR gene for specific identification of Bacillus anthracis. J Clin Microbiol. 2005; 43(4):1995-7.
[47]
Antonation KS, Grützmacher K, Dupke S, Mabon P, Zimmermann F, Lankester F, Peller T, Feistner A, Todd A, Herbinger I, de Nys HM, Muyembe-Tamfun JJ, Karhemere S, Wittig RM, Couacy-Hymann E, Grunow R, Calvignac-Spencer S, Corbett CR, Klee SR, Leendertz FH. Bacillus cereus Biovar Anthracis Causing Anthrax in Sub-Saharan Africa-Chromosomal Monophyly and Broad Geographic Distribution. PLoS Negl Trop Dis. 2016; 10(9):e0004923.
[48]
Klee SR, Ozel M, Appel B, Boesch C, Ellerbrok H, Jacob D, Holland G, Leendertz FH, Pauli G, Grunow R, Nattermann H. Characterization of Bacillus anthracis-like bacteria isolated from wild great apes from Cote d'Ivoire and Cameroon. J Bacteriol. 2006; 188(15):5333-44.
[49]
Marston CK, Ibrahim H, Lee P, Churchwell G, Gumke M, Stanek D, Gee JE, Boyer AE, Gallegos-Candela M, Barr JR, Li H, Boulay D, Cronin L, Quinn CP, Hoffmaster AR. Anthrax Toxin-Expressing Bacillus cereus Isolated from an Anthrax-Like Eschar. PLoS One. 2016; 11(6):e0156987.
[50]
Brangsch H, Golovko A, Pinchuk N, Deriabin O, Kyselova T, Linde J, Melzer F, Elschner MC. Molecular Typing of Ukrainian Bacillus anthracis Strains by Combining Whole-Genome Sequencing Techniques. Microorganisms. 2022; 10(2):461.
[51]
Juergensmeyer MA, Gingras BA, Restaino L, Frampton EW. A selective chromogenic agar that distinguishes Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. J Food Prot. 2006; 69(8):2002-6.
[52]
Rohde A, Papp S, Feige P, Grunow R, Kaspari O. Development of a novel selective agar for the isolation and detection of Bacillus anthracis. J Appl Microbiol. 2020; 129(2):311-8.
[53]
Owen MP, Schauwers W, Hugh-Jones ME, Kiernan JA, Turnbull PC, Beyer W. A simple, reliable M'Fadyean stain for visualizing the Bacillus anthracis capsule. J Microbiol Methods. 2013; 92(3):264-9.
[54]
Stopa PJ. The flow cytometry of Bacillus anthracis spores revisited. Cytometry. 2000; 41(4):237-44.
[55]
Zahavy E, Fisher M, Bromberg A, Olshevsky U. Detection of frequency resonance energy transfer pair on double-labeled microsphere and Bacillus anthracis spores by flow cytometry. Appl Environ Microbiol. 2003; 69(4):2330-9.
[56]
Cohen N, Zahavy E, Zichel R, Fisher M. An internal standard approach for homogeneous TR-FRET immunoassays facilitates the detection of bacteria, biomarkers, and toxins in complex matrices. Anal Bioanal Chem. 2016; 408(19):5179-88.
[57]
Straub T, Baird C, Bartholomew RA, Colburn H, Seiner D, Victry K, Zhang L, Bruckner-Lea CJ. Estimated copy number of Bacillus anthracis plasmids pXO1 and pXO2 using digital PCR. J Microbiol Methods. 2013; 92(1):9-10.
[58]
Zasada AA. Detection and Identification of Bacillus anthracis: From Conventional to Molecular Microbiology Methods. Microorganisms. 2020; 8(1):125.
[59]
Yu H. Comparative studies of magnetic particle-based solid phase fluorogenic and electrochemiluminescent immunoassay. J Immunol Methods. 1998; 218(1-2):1-8.
[60]
Zasada AA, Formińska K, Zacharczuk K, Jacob D, Grunow R. Comparison of eleven commercially available rapid tests for detection of Bacillus anthracis, Francisella tularensis and Yersinia pestis. Lett Appl Microbiol. 2015; 60(5):409-13.
[61]
Limanskaya OYu, Murtazaeva LA, Klee S, Limanskii A. Detection of causative agent of anthrax by real-time polymerase chain reaction. Biotechnology. 2012; 5(5):65-71
[62]
Avberšek J, Mićunović J, Cociancich V, Paller T, Kušar D, Zajc U, Ocepek M, Špičić S, Duvnjak S, Pate M. A Suggested Diagnostic Approach for Sporadic Anthrax in Cattle to Protect Public Health. Microorganisms. 2021; 9(8):1567.
[63]
Rohde A, Papp S, Feige P, Grunow R, Kaspari O. Development of a novel selective agar for the isolation and detection of Bacillus anthracis. J Appl Microbiol. 2020; 129(2):311-18.
[64]
Kolton CB, Marston CK, Stoddard RA, Cossaboom C, Salzer JS, Kozel TR, Gates-Hollingsworth MA, Cleveland CA, Thompson AT, Dalton MF, Yabsley MJ, Hoffmaster AR. Detection of Bacillus anthracis in animal tissues using InBios active anthrax detect rapid test lateral flow immunoassay. Lett Appl Microbiol. 2019; 68(6):480-4.
[65]
Kim J, Gedi V, Lee SC, Cho JH, Moon JY, Yoon MY. Advances in Anthrax Detection: Overview of Bioprobes and Biosensors. Appl Biochem Biotechnol. 2015; 176(4):957-77.