Biopolym. Cell. 2020; 36(5):358-370.
http://dx.doi.org/10.7124/bc.000A39
Біоорганічна хімія
Флуоресцентні кон’югати D-глюкозаміну з 3-тіазолілкумаринами: синтез, характеризація та потенційне застосування як реагентів для візуалізації клітин
1Кузів Я. Б., 1Новосильна О. В., 1Дубей І. Я.
  1. Інститут молекулярної біології і генетики НАН України
    Вул. Академіка Заболотного, 150, Київ, Україна, 03143

Abstract

Мета. Синтез і вивчення кон’югатів глюкозамін-кумарин як можливих реагентів для флуоресцентної візуалізації клітин. Методи. Органічний синтез, ЯМР, оптична спектроскопія, конфокальна мікроскопія. Результати. Кон’югати D-глюкозаміну з 7-заміщеними 3-тіазолілкумаринами отриміано з виходами 55-82% реакцією NHS-активованих естерів карбокси-модифікованих барвників з аміногрупою цукру. За допомогою ЯМР визначено аномерну конфігурацію продуктів. Досліджено їхні спектрільні властивості в метанолі й фосфатному буфері. Попередньо оцінено можливість використання кон’югатів для фарбування клітин. Висновки. Тіазолілкумарин-мічені глюкозаміни з яскравою блакитною емісією – перспективні реагенти для візуалізації клітин.
Keywords: кумарини, глюкозамін, кон’югати, флуоресценція, конфокальна мікроскопія
Повний текст: (PDF, англійською)

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