Biopolym. Cell. 2020; 36(3):182-196.
http://dx.doi.org/10.7124/bc.000A29
Огляди
Системи тривимірного культивування клітин
1, 2Сукач О. М., 1Шевченко М. В.
  1. Інститут проблем кріобіології і кріомедицини НАН України
    23, Переяслівська вул., Харків, Україна, 61015
  2. Харківський національний педагогічний університет імені Г. С. Сковороди
    вул. Алчевських, 29, Харків, Україна, 61002

Abstract

В огляді обговорюються особливості тривимірних систем культивування клітин на носіях (скафолдах) і без носіїв. Скафолди використовують з метою імітації позаклітинного матриксу, а також для відтворення природного фізичного і структурного мікрооточення клітин, подібного живій тканині. В огляді розглядаються типи скафолдів (жорсткі і гелевидні, природні та штучні, що розкладаються і не розкладаються), їх характеристики, переваги, недоліки та особливості заселення клітинами. Обговорюється використання в якості скафолдів децеллюлялізованих і девіталізованих органів і тканин. Також в огляді розглядається безматриксне культивування клітин у складі тривимірних багатоклітинних структур – сфероїдів. Обговорюється структура і біологія сфероїдів. Розглядаються особливості утворення сфероїдів в статичних (самозбірка) і динамічних (під впливом зовнішніх сил) умовах культивування. Обговорюється роль розміру сфероїдів для виживання клітин.
Keywords: клітини, 3D клітинна культура, скафолди, сфероїди
Повний текст: (PDF, англійською)

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