Biopolym. Cell. 2020; 36(1):36-47.
http://dx.doi.org/10.7124/bc.000A1F
Методи
Пряме мічення нуклеозидів 3-тіазолілкумариновими флуоресцентними барвниками
1Кузів Я. Б., 1Дубей І. Я.
  1. Інститут молекулярної біології і генетики НАН України
    Вул. Академіка Заболотного, 150, Київ, Україна, 03143

Abstract

Мета. Отримання й дослідження нуклеозидів, мічених флуорофорами на основі кумарину без попередньої функціоналізації. Методи. Органічний синтез, адсорбційна та флуоресцентна спектроскопія. Результати. Проведене пряме мічення 2’-дезоксинуклеозидів карбокси-модифікованими тіазолілкумаринами. Кон’югати отримано реакцією гідроксибензотріазольних активованих естерів барвників з 5’-гідроксилом нуклеозиду в присутності основи або з аміногрупою цитозину. Досліджено їхні оптичні властивості в метанолі й фосфатному буфері. Висновки. N- та O-ацилювання піримідинових нуклеозидів похідними кумаринів дозволило отримати кон’югати з яскравою блакитною емісією.
Keywords: кумарини, нуклеозиди, флуоресцентне мічення, активовані естери
Повний текст: (PDF, англійською)

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