Biopolym. Cell. 2019; 35(4):268-287.
http://dx.doi.org/10.7124/bc.000A0A
Структура та функції біополімерів
Аналіз інтерактому eEF1Bγ в ядерній фракції клітин аденокарциноми легені людини A549
1Капустян Л. М., 1Лисецький І. Л., 1Бондарчук Т. В., 1Новосильна О. В., 1Негруцький Б. С.
  1. Інститут молекулярної біології і генетики НАН України
    Вул. Академіка Заболотного, 150, Київ, Україна, 03143

Abstract

Мета. Виявити нові функції фактора елонгації трансляції eEF1Bgamma (eEF1Bγ) в ядерній фракції клітин карциноми легені людини А549. Методи. Білки-партнери eEF1Bγ у ядерній фракції клітин аденокарциноми легені людини А549 були ідентифіковані за допомогою ко-іммунопреципітації із наступною рідинною хроматографією та тандемною мас-спектрометрією (LC-MS/MS). Білкові мережі, до яких входить локалізований у ядрі білок eEF1Bγ, визначали за допомогою програми Cytoscape 3.2.0 із плагіном MCODE. Додатковий аналіз партнерів ядерного eEF1Bγ проводили за допомогою бази даних Mapofthecell. Результати. Ідентифіковано 234 білки, що взаємодіють із eEF1Bγ в ядерній фракції клітин А549. Мережі білок-білкових взаємодій, до яких залучені ці білки, були проаналізовані за допомогою двох біоінформатичних підходів. Висновки. Висунуто передбачення, що сплайсинг пре-мРНК та регуляція стабільності мРНК можуть бути основними процесами, у яких бере участь ядерно локалізований eEF1Bγ. Під час карциногенезу частина молекул eEF1Bγ залишає локалізований у цитоплазмі комплекс eEF1B і переходить до ядра, де регулює кількість специфічних мРНК через контроль сплайсингу відповідних пре-мРНК та вплив на стабільність мРНК.
Keywords: eEF1Bγ, білок-білкові взаємодії, ядро, клітини А549
Повний текст: (PDF, англійською)

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