Biopolym. Cell. 2016; 32(3):203-221.
http://dx.doi.org/10.7124/bc.000921
Геноміка, транскриптоміка та протеоміка
Транскрипційна та постранскрипційна регуляція гена адапторного/скафолдного білка ITSN1
1Кропивко С. В., 1Губар О. С., 1Грязнова Т. А., 1Мордерер Д. Е., 1Герасимчук Д. О., 2Сивак Л. А., 2Грабовий О. М., 1Риндич А. В.
  1. Інститут молекулярної біології і генетики НАН України
    Вул. Академіка Заболотного, 150, Київ, Україна, 03680
  2. Національний інститут раку
    вул. Ломоносова, 33/43, Київ, Україна, 03022

Abstract

ITSN1 – адаптерний/скафолдний білок який приймає участь у різноманітних фізіологічних та патологічних клітинних процесах. Він має складну регуляцію експресії та багаточисельні білки партнери. Мета. Характеристика функціонування та регуляції експресії ITSN1 має важливе значення для розуміння ролі ITSN1 в житті клітини. Методи. Біоінформатичний аналіз, напівкількісний аналіз експресії за допомогою ЗТ-ПЛР, іммунопреципітація. Результати. Описано та проаналізовано промоторні регіони ITSN1, детектовано альтернативно сплайсовані ізоформи ITSN1 на рівні мРНК та білка в різних зразках раку. За допомогою різноманітних біоінформатичних серверів виявлено сайти зв’язування з мікроРНК, проаналізовано сайти серин-, треонін- та тирозин-фосфорилювання білка ITSN1. Висновки. Отримано нові дані про експресію ITSN1 при патологіях. Показано можливість регуляції экспресії ITSN1 за допомогою мікроРНК та ролі фосфорилювання серину, треоніну и тирозину в регуляції взаємодії ITSN1 з білками партнерами.
Keywords: ITSN1, двонаправлений промотор, альтернативний сплайсинг, мікроРНК, фосфорилювання
Повний текст: (PDF, англійською)

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