Нові партнери скафолдного білка TKS4

Кропивко, С. В.

doi:10.7124/bc.0008FC

Biopolym. Cell. 2015; 31(5):395-401.

http://dx.doi.org/10.7124/bc.0008FC

Короткі повідомлення

Нові партнери скафолдного білка TKS4

1Кропивко С. В.

Інститут молекулярної біології і генетики НАН України
Вул. Академіка Заболотного, 150, Київ, Україна, 03680

Abstract

Скафолд TKS4 бере участь у формуванні інвадоподій, продукції активних форм кисню (ROS) в ракових клітинах та інших клітинних процесах. Мета. Ідентифікувати нових партнерів TKS4, задіяних в перебудовах актинового цитоскелету та ендо-/екзоцитозі. Методи. In vitro GST pull-down assay. Результати. Показано, що SH3 домени TKS4 взаємодіють з білками-реорганізаторами актинового цитоскелету N-WASP та CR16, а також з DNM2, SYNJ1 та OPHN1, які задіяні в ендо-/екзоцитозі. Проте взаємодії WIP, WIRE, SHIP2, RhoU, RhoV та NUMB, з SH3 доменами TKS4 не знайдено. Висновки. SH3 домени TKS4 взаємодіють з білками N-WASP, DNM2, SYNJ1, OPHN1 та слабко з CR16 in vitro.

Keywords: TKS4, адаптерні/скафолдні білки, актиновий цитоскелет, ендо-/екзоцитоз

Повний текст: (PDF, англійською)

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