Biopolym. Cell. 2014; 30(5):358-364.
http://dx.doi.org/10.7124/bc.0008B2
Геноміка, транскриптоміка та протеоміка
Чутливість дріжджів Sассharomyces cerevisiae, дефектних за різними ділянками сигнального шляху TOR, до карбонільного/оксидативного стресу
1Валішкевич Б. В.
  1. Прикарпатський Національний Університет імені Василя Стефаника
    вул. Шевченка, 57, Івано-Франківськ, Україна, 76025

Abstract

Мета. Дослідити вплив карбонільного/оксидативного стресу, індукованого гліоксалем, метилгліоксалем та пероксидом водню, на виживання штамів S. cerevisiae, дефектних за різними ділянками TOR-сигнального шляху, за умов їхнього росту у середовищі із глюкозою чи фруктозою. Методи. Оцінка репродуктивної здатності методом визначення кількості колонієутворювальних одиниць. Результати. Показано, що у певних концентраціях дія вищезазначених агентів викликає підвищення рівня виживання. Це свідчить про наявність горметичного ефекту. Висновки. Шлях TOR залучений до горметичного ефекту всіх використаних токсикантів, проте наявність даного ефекту є специфічною для кожного штаму та залежить від типу вуглеводу у середовищі інкубації.
Keywords: Saccharomyces cerevisiae, глюкоза, фруктоза, сигнальний шлях TOR, карбонільний/оксидативний стрес
Повний текст: (PDF, англійською)

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