Biopolym. Cell. 2012; 28(4):261-266 .
http://dx.doi.org/10.7124/bc.000058
Огляди
Генетична трансформація мітохондрій: методичний вибір між класичними біотехнологічними підходами та природною компетенцією органел
1Кулінченко М. В., 2Дитриш А., 1Константинов Ю. М.
  1. Сибірський інститут фізіології і біохімії рослин СО РАН
    вул. Лермонтова 132, Іркутськ, Російська Федерація, 664033
  2. Інститут молекулярної біології рослин, Університета Страсбурга
    вул. Генерала Зіммера, 12, Страсбург, Франція, 67084

Abstract

Незважаючи на досить переконливі докази існування горизонтального перенесення генів у рослинні мітохондрії, у цій області досліджень залишається чимало невирішених питань, зокрема, не відомий молекулярний механізм горизонтального перенесення генів в органели. На сьогодні не до кінця вивчено низку питань як трансмембранного перенесення, так і умов інтеграції гетерологічної ДНК у мітохондріальний геном виду-реципієнта. Виявлені в мітохондріях багатьох видів вищих рослин кільцеві і, особливо, лінійні плазміди, з одного боку, є зручним інструментом для вивчення механізмів трансмембранного перенесення ДНК і, з іншого, – можуть слугувати основою для конструювання мітохондріальних векторів інтегративного типу.
Keywords: трансформація мітохондрій, імпорт ДНК, мітохондріальні плазміди, мітохондріальна мембрана
Повний текст: (PDF, англійською)

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