Biopolym. Cell. 2010; 26(5):360-372.
http://dx.doi.org/10.7124/bc.00016A
Огляди
Поліморфізм ДНК і білково-нуклеїнове впізнавання
1Борискіна О. П., 1Ткаченко М. Ю., 1Шестопалова Г. В.
  1. Інститут радіофізики і електроніки ім. А. Я. Усикова НАН України
    вул. Академіка Проскури, 12, Харків, Україна, 61085

Abstract

Розшифрування молекулярних механізмів упізнавання білками конкретних послідовностей ДНК – одна із основних задач сучасної біології. Огляд присвячено результатам статистичного аналізу структурних баз даних, отриманих різними групами дослідників при вивченні конформаційних особливостей фрагментів вільної ДНК і ДНК у комплексах з білками. Такі дані використовують при дослідженні механізмів білково-нуклеїнового впізнавання. Аналіз літературних джерел дозволив зробити наступні узагальнення. Здатність подвійної спіралі ДНК набувати альтернативних конформацій, у тому числі сахарофосфатним остовом, є «внутрішньою» властивістю, яка притаманна визначеним послідовностям ДНК. Подібні конформаційні перебудови можуть слугувати потенційним джерелом прояву унікальних особливостей геометрії різних динуклеотидних фрагментів і/або окремих нуклеотидів та призводити до порушення стекінгу основ і/або змін площ доступної поверхні атомів, а також бути критеріями впізнавання білками специфічних послідовностей ДНК. Зміни фізичних властивостей, які залежать від структури ДНК, серед яких полярно-неполярний профіль і електростатичний потенціал борозенок, також можуть використовуватися білками для впізнавання конкретного сайта ДНК.
Keywords: білково-нуклеїнове впізнавання, поліморфізм ДНК, сахарофосфатний остов ДНК, альтернативні конформації
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