Biopolym. Cell. 2009; 25(2):115-119.
http://dx.doi.org/10.7124/bc.0007D3
Молекулярна та клітинна біотехнології
Визначення локалізації Methylobacterium radiotolerans IMBG290 у тканинах картоплі методом гібридизації in situ
1Подоліч О. В., 2Овчаренко Л. П., 2Козировська Н. О., 3Пірттіла А. М.
  1. Інститут агроекології і природокористування НААН України
    вул. Метрологічна 12, Київ, Україна, 03680
  2. Інститут молекулярної біології і генетики НАН України
    Вул. Академіка Заболотного, 150, Київ, Україна, 03680
  3. Біологічний факультет Університету Оулу
    Ліннанмаа, PO Box 3000, FIN-90014 Оулу, Фінляндія

Abstract

Новий штам рожево-пігментованої факультативної метилотрофної бактерії M. radiotolerans IMBG290, виділений раніше з культури тканин картоплі після обробки бактерією Pseudomonas fluorescens IMBG163, визначено в тканинах картоплі методом гібридизації in situ (ISH/FISH). Наявність рибосомної 16S РНК спостерігали в листі та стеблах пробіркових рослин, проте її не знайдено в корінні картоплі. У рослин, оброблених M. radiotolerans IMBG290, гібридизаційний сигнал виявився сильнішим, ніж у контрольних (необроблених) рослин.
Keywords: гібридизація in situ, Methylobacterium radiotolerans IMBG290, живці картоплі
Повний текст: (PDF, англійською)

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