Biopolym. Cell. 2008; 24(1):35-40.
Клітинна біологія
Субклітинна локалізація S6K1 і S6K2 форм рибосомної
протеїнкінази S6 у первинній моношаровій культурі
тиреоцитів щурів
- Інститут молекулярної біології і генетики НАН України
Вул. Академіка Заболотного, 150, Київ, Україна, 03680
Abstract
Імунохімічно визначено субклітинну локалізацію S6K1 і S6K2 у тканині та моношаровій культурі щитовидної залози щурів. Встановлено, що в тканині щитовидної залози S6K1 і S6K2 локалізуються переважно в цитоплазмі клітин. При розпластуванні фолікулів з’являлася позитивна реакція в ядрах тиреоцитів. Такий перерозподіл не пов’язаний прямо з появою проліферуючих Ki-67 позитивних клітин. У той же час відмічено кореляцію між появою S6K1, S6K2 позитивних ядер і зменшенням вмісту тиреоглобулину у культивованх клітинах. Таким чином, отримані результати свідчать про те, що зниження функціональної активності тиреоцитів внаслідок втрати фолікулярної організації супроводжується зміною субклітинної локалізації S6K1 і S6K2.
Keywords: рибосомна протеїнкіназа S6, тиреоцити, первинна культура
Повний текст: (PDF, англійською)
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