Biopolym. Cell. 2004; 20(6):479-492.
Структура та функції біополімерів
Особливості процесу самоасоціації дезоксигексануклеотиду 5'-
d(GpCpApTpGp) і його комплексоутворення з антрацикліновим
антибіотиком дауноміцином у водному розчині
- Севастопольський національний технічний университет
вул. Університетська, 33, Севастополь, Україна, 99053 - Беркбек колледж Лондонского университета
Малет-стрит, Лондон WC1E 7НХ, Великобритания
Abstract
Методами одно- і двовимірної кореляційної 1Н-ЯМР спектроскопи (2M-TOCSY і 2M-NOESY) досліджено самоасоціацію самокомплементарного дезоксигексануклеотиду 5'-d(GpCpApTpGp) і його комплексоутворення з протипухлинним антибіотиком дауноміцином у водному розчині. Визначено концентраційні і температурні залежності хімічних зсувів необмінних протонів дезоксигексануклеотиду і дауноміцину, за якими розраховано рівноважні константи і термодинамічні
параметри (ΔН і ΔS) реакцій утворення дуплексу d(GCATGC) і комплексів гексамеру з антибіотиком. На основі аналізу змін протонних хімічних зсувів гексамеру в області низьких концентрацій і при порівняно невисоких температурах зроблено припущення про формування послідовністю d(GCATGC) компактної структури (наприклад, подібній до шпильки) у водному розчині. Побудовано просторові структури итильки та інтеркаляцийного комплексу дауноміцину з дуплексом гексамеру d(GCATGC) методами молекулярної механіки з використанням програми X-PLOR. Проведено порівняльний аналіз
параметрів утворення комплексів антибіотика дауноміцину з дезоксигексануклеотидами різної послідовності основ у ланцюзі.
Повний текст: (PDF, російською)
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