Biopolym. Cell. 2003; 19(3):211-215.
http://dx.doi.org/10.7124/bc.000651
Огляди
Використання таргетингу для вирішення проблем біології розвитку
1Анопрієнко О. В., 1Євсіков О. В.
  1. Інститут молекулярної біології і генетики НАН України
    Вул. Академіка Заболотного, 150, Київ, Україна, 03680

Abstract

В останні роки центр ваги ембріологічних досліджень змістився на вивчення ролі окремих генів в онтогенезі. Активне впровадження нових молекулярно-біологічних підходів, що дозволяють досліджувати функції генів та динаміку їхньої експресії в процесі розвитку організму, розпочалося з розробки технології культивування миишчих ES-клітин. В огляді розглянуто загальні принципи деяких сучасних методів — спрямованої модифікації генів за допомогою гомологічної рекомбінації, Cre–LoxP-системи сайт-спрямованої рекомбінації, стратегії «gene-trapping», оцінено їхні можли­вості та перспективи використання.
Повний текст: (PDF, українською)

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