Biopolym. Cell. 1999; 15(5):367-373.
http://dx.doi.org/10.7124/bc.000532
Структура та функції біополімерів
Синтез та вивчення антисенсових олігонуклеотидів, модифікованих імідазофеназиновими нуклеозидами
1Макітрук В. Л., 1Шаламай А. С., 2Дубей І. Я., 2Федоряк Д. М.
  1. Інститут молекулярної біології і генетики НАН України
    Вул. Академіка Заболотного, 150, Київ, Україна, 03680
  2. Інститут біоорганічної хімії та нафтохімії НАН України
    вул. Мурманська, 1, Київ, Україна, 02094

Abstract

Н-фосфонатним твердофазным методом отримано модифіковані імідазофеназиновими нуклеози­дами по 3'-, 5'- та внутрішніх положеннях олігонуклеотиди, комплементарні до ділянок рибосомного оперона, 16S рРНК мікоплазм та Escherichia coli. Показано, шр нуклеозиди імідазо[4,5-b]феназину та його 2-метального аналога, діючи як ефективні інтеркалятори, суттєво впливають на стабільність комплементарних дуплексів і тим самим підвищують антисенсову активність модифікованих олігонуклеотидних послідовностей.
Повний текст: (PDF, українською)

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