Biopolym. Cell. 1999; 15(1):28-37.
Структура та функції біополімерів
Рекомбінантна ДНК pGins, що містить експресуючий ген препроінсуліну людини, не виявляє мутагенной активності
1Лукаш Л. Л., 1Подольська С. В., 1Євсеєнко А. А., 1Сухорада О. М., 1Неборачко Л. М., 1Варзанова І. С., 1Кочубей Т. П.
  1. Інститут молекулярної біології і генетики НАН України
    Вул. Академіка Заболотного, 150, Київ, Україна, 03680

Abstract

На відміну від мутагенних рекомбінантних плазмід pBR322ins та рAins, ДНК pGins у концентрації 10 мкг/мл не викликає підвищення частоти генних мутацій та хромосомних аберацій у соматичних клітинах ссавців. Відсутність мутагенного ефекту pGins підтверджено у різних тест-системах in vitro та in vivo. Ймовірно, що це зумовлено підсиленням експресії та функцій гена препроінсуліну під впливом вірусних регуля­торних елементів. Показано, шр обробка клітин ссавців, що культивуються, препаратом інсуліну захищає їх від мутаген­ної дії рекомбінантної плазміди рНВ320, яка містить геном вірусу гепатиту В. Одержані дані свідчать про участь гена препроінсуліну, що експресу ється, у підтриманні рівня гене­тичної стабільності або мінливості клітинних популяцій.

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