Biopolym. Cell. 1998; 14(4):349-359.
Структурно-функціональне дослідження тирозил-тРНК синтетази ссавців
- Інститут молекулярної біології і генетики НАН України
Вул. Академіка Заболотного, 150, Київ, Україна, 03680
Abstract
В огляді представлено основні, результати структурно-функціонального дослідження тирозил тРНК синтетази ссавців (КФ 6-1.1.1). Розглянуто множинність молекулярних форм ферменту. Вивчено структуру активного центра синтетази методами хімічних модифікацій і показано суттєву роль залишків лізину, гістидину і цистеїну. Продемонстровано, що до взаємодії з основною формою тирозил-тРНК синтетази залучається антикодон гомологічної тРНКTyr . Встановлено специфічні конформаційні зміни ферменту при взаємодії з субстратами, в тому числі з тРНК ; виявлено конформаційну адаптацію центрів зв'язування тРНК і АТР. На основі отриманих даних запропоновано динамічну модель функціонування синтетази. Визначено первинну структуру тирозил-тРНК синтетази бика шляхом клонування і секвенування к ДНК. Запропоновано модульну організацію ферменту: каталітична частина, яка складається зі згортки Россмана та α-спірального домену, з'єднана з цитокін-подібним некаталітинним модулем, спорідненим з РНК. Запропоновано гіпотезу стосовно утворення ізольованого С-домену внаслідок обмеженого протеолізу після активації внутрішньоклітинних проте аз і проявлення цим доменом цитокінподібної активності, аналогічно цитокіну EMAP IІ.
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