Biopolym. Cell. 1998; 14(2):163-172.
Синтез та використання дисульфідного Н-фосфонатного реагента для 3 ' - та/або 5'-мічення олігонуклеотидів через меркаптоалкільний лінкер
1Дубей І. Я., 1Федоряк Д. М.
  1. Інститут біоорганічної хімії та нафтохімії НАН України
    вул. Мурманська, 1, Київ, Україна, 02094


Описано синтез нового дисульфідного Н-фосфонатного реагента для твердофазного введення в олігонуклеотиди меркаптопропільних груп по одному чи двох кінцях. З використанням цього реагента було синтезовано З'- та 5'-дисульфідні похідні олігонуклеотидів. Дисульфідні зв'язки розщеплено дитіотреїтолом і тіольні групи, що утворилися, модифіковано йодацетамідофлюоресцеїном. З високим виходом одержано оліго-Т15, які несуть один чи два залишки флюоресиеіну на У- і 5 -кінцях.


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