Biopolym. Cell. 1994; 10(6):36-44.
Сучасні аспекти генетики бактерій роду Campylobacter
1Кірік Д. Л.
  1. Інститут епідеміології та інфекційних хвороб ім. Л. В. Громашевського АМН України
    вул. Амосова, 5, Київ, Україна, 03038

Abstract

В аналкичному огляді літератури представлено сучасні дані про генетичну організацю кампілобактерій – збудників ГКІ у людини. Узагальнено відомості про молекулярні основи патогенності кампілобактерій: наявність жгутиків, ентеротоксина, головного зовнішнього мембранного білка та поверхневого білка. Гени, що кодують резистентність кампілобактерій до антибютиків (Km, Тс, Cm), клоновано i визначеноіхню нуклеотидну послідовність. Стійкість до вказаних антибютиюв має плазмідний характер, i деякі плазміди кодують більше ніж одну детермінанту. Проаналізовано механізми генетичного обміну у кампілобактерій (трансдукція, транскон'югація, транформaцiя). На сьогодні визначено розміри геному i сконструйовано пари хромосом у деяких кампілобактерій. За кордоном показано можливість використання методів молекулярної біології для практичних епідеміологічих дocлiджeнь: гель-електрофореза в електричному полі, рестрикщйно-ендонуклеазного аналізу ДНК, багатолокусного ензимного електрофореза, iмyнoблoтингa, риботипування, плазмщного аналізу i полімеразної ланцюгової реакції.

References

[1] Aguero-Rosenfeld ME, Yang XH, Nachamkin I. Infection of adult Syrian hamsters with flagellar variants of Campylobacter jejuni. Infect Immun. 1990;58(7):2214-9.
[2] Bannam TL, Rood JI. Relationship between the Clostridium perfringens catQ gene product and chloramphenicol acetyltransferases from other bacteria. Antimicrob Agents Chemother. 1991;35(3):471-6.
[3] Black RE, Levine MM, Clements ML, Hughes TP, Blaser MJ. Experimental Campylobacter jejuni infection in humans. J Infect Dis. 1988;157(3):472-9.
[4] Blaser MJ, Gotschlich EC. Surface array protein of Campylobacter fetus. Cloning and gene structure. J Biol Chem. 1990;265(24):14529-35.
[5] Brosius J. Toxicity of an overproduced foreign gene product in Escherichia coli and its use in plasmid vectors for the selection of transcription terminators. Gene. 1984;27(2):161-72.
[6] Bruce D, Hookey JV, Waitkins SA. Numerical classification of campylobacters by DNA-restriction endonuclease analysis. Zentralbl Bakteriol Mikrobiol Hyg A. 1988;269(3):284-97.
[7] Caldwell MB, Guerry P, Lee EC, Burans JP, Walker RI. Reversible expression of flagella in Campylobacter jejuni. Infect Immun. 1985;50(3):941-3.
[8] Calva E, Torres J, Vázquez M, Angeles V, de la Vega H, Ruíz-Palacios GM. Campylobacter jejuni chromosomal sequences that hybridize to Vibrio cholerae and Escherichia coli LT enterotoxin genes. Gene. 1989;75(2):243-51.
[9] Chan VL, Bingham HL. Complete sequence of the Campylobacter jejuni glyA gene encoding serine hydroxymethyltransferase. Gene. 1991;101(1):51-8.
[10] Chan VL, Bingham H, Kibue A, Nayudu PR, Penner JL. Cloning and expression of the Campylobacter jejuni glyA gene in Escherichia coli. Gene. 1988;73(1):185-91.
[11] Chang W, Ogg JE. Transduction in vibrio fetus. Am J Vet Res. 1970;31(5):919-24.
[12] Chang N, Taylor DE. Use of pulsed-field agarose gel electrophoresis to size genomes of Campylobacter species and to construct a SalI map of Campylobacter jejuni UA580. J Bacteriol. 1990;172(9):5211-7.
[13] Clayton CL, Pallen MJ, Kleanthous H, Wren BW, Tabaqchali S. Nucleotide sequence of two genes from Helicobacter pylori encoding for urease subunits. Nucleic Acids Res. 1990;18(2):362.
[14] Evans DG, Evans DJ Jr, Moulds JJ, Graham DY. N-acetylneuraminyllactose-binding fibrillar hemagglutinin of Campylobacter pylori: a putative colonization factor antigen. Infect Immun. 1988;56(11):2896-906.
[15] Fischer SH, Nachamkin I. Common and variable domains of the flagellin gene, flaA, in Campylobacter jejuni. Mol Microbiol. 1991;5(5):1151-8.
[16] Fox JG, Taylor NS, Penner JL, Shames B, Gurgis RV, Tomson FN. Investigation of zoonotically acquired Campylobacter jejuni enteritis with serotyping and restriction endonuclease DNA analysis. J Clin Microbiol. 1989;27(11):2423-5.
[17] Giesendorf BA, van Belkum A, Koeken A, Stegeman H, Henkens MH, van der Plas J, Goossens H, Niesters HG, Quint WG. Development of species-specific DNA probes for Campylobacter jejuni, Campylobacter coli, and Campylobacter lari by polymerase chain reaction fingerprinting. J Clin Microbiol. 1993;31(6):1541-6.
[18] Goossens H, Pot B, Vlaes L, Van den Borre C, Van den Abbeele R, Van Naelten C, Levy J, Cogniau H, Marbehant P, Verhoef J, et al. Characterization and description of "Campylobacter upsaliensis" isolated from human feces. J Clin Microbiol. 1990;28(5):1039-46.
[19] Grajewski BA, Kusek JW, Gelfand HM. Development of a bacteriophage typing system for Campylobacter jejuni and Campylobacter coli. J Clin Microbiol. 1985;22(1):13-8.
[20] Guerry P, Alm RA, Power ME, Logan SM, Trust TJ. Role of two flagellin genes in Campylobacter motility. J Bacteriol. 1991;173(15):4757-64.
[21] Guerry P, Logan SM, Thornton S, Trust TJ. Genomic organization and expression of Campylobacter flagellin genes. J Bacteriol. 1990;172(4):1853-60.
[22] Harris LA, Logan SM, Guerry P, Trust TJ. Antigenic variation of Campylobacter flagella. J Bacteriol. 1987;169(11):5066-71.
[23] Kim NW, Chan VL. Isolation ahd characterization of the ribosomal RNA genes ofCampylobacter jejuni. Curr Microbiol. 1989;19(4):247–52.
[24] Labigne-Roussel A, Courcoux P, Tompkins L. Gene disruption and replacement as a feasible approach for mutagenesis of Campylobacter jejuni. J Bacteriol. 1988;170(4):1704-8.
[25] Lee EC, Walker RI, Guerry P. Expression of Campylobacter genes for proline biosynthesis in Escherichia coli. Can J Microbiol. 1985;31(11):1064-7.
[26] Logan SM, Trust TJ, Guerry P. Evidence for posttranslational modification and gene duplication of Campylobacter flagellin. J Bacteriol. 1989;171(6):3031-8.
[27] Manavathu EK, Fernandez CL, Cooperman BS, Taylor DE. Molecular studies on the mechanism of tetracycline resistance mediated by Tet(O). Antimicrob Agents Chemother. 1990;34(1):71-7.
[28] Manavathu EK, Hiratsuka K, Taylor DE. Nucleotide sequence analysis and expression of a tetracycline-resistance gene from Campylobacter jejuni. Gene. 1988;62(1):17-26.
[29] Miller JF, Dower WJ, Tompkins LS. High-voltage electroporation of bacteria: genetic transformation of Campylobacter jejuni with plasmid DNA. Proc Natl Acad Sci U S A. 1988;85(3):856-60.
[30] Morooka T, Umeda A, Amako K. Motility as an intestinal colonization factor for Campylobacter jejuni. J Gen Microbiol. 1985;131(8):1973-80.
[31] Morrison DA, Jaurin B. Streptococcus pneumoniae possesses canonical Escherichia coli (sigma 70) promoters. Mol Microbiol. 1990;4(7):1143-52.
[32] Mullis KB. The unusual origin of the polymerase chain reaction. Sci Am. 1990;262(4):56-61, 64-5.
[33] Nachamkin I, Xiao-He Yang. Isoelectric focusing of Campylobacter jejuni flagellin: microheterogeneity and restricted antigenicity of charged species with a monoclonal antibody. FEMS Microbiol Lett. 1988;49(2):235–8.
[34] Nachamkin I, Yang XH. Human antibody response to Campylobacter jejuni flagellin protein and a synthetic N-terminal flagellin peptide. J Clin Microbiol. 1989;27(10):2195-8.
[35] Nachamkin I, Bohachick K, Patton CM. Flagellin gene typing of Campylobacter jejuni by restriction fragment length polymorphism analysis. J Clin Microbiol. 1993;31(6):1531-6.
[36] Newell DG. Monoclonal antibodies directed against the flagella of Campylobacter jejuni: cross-reacting and serotypic specificity and potential use in diagnosis. J Hyg (Lond). 1986;96(3):377-84.
[37] Newell DG, McBride H, Dolby JM. Investigations on the role of flagella in the colonization of infant mice with Campylobacter jejuni and attachment of Campylobacter jejuni to human epithelial cell lines. J Hyg (Lond). 1985;95(2):217-27.
[38] Nuijten PJ, Bartels C, Bleumink-Pluym NM, Gaastra W, van der Zeijst BA. Size and physical map of the Campylobacter jejuni chromosome. Nucleic Acids Res. 1990;18(21):6211-4.
[39] Nuijten PJ, Bleumink-Pluym NM, Gaastra W, van der Zeijst BA. Flagellin expression in Campylobacter jejuni is regulated at the transcriptional level. Infect Immun. 1989;57(4):1084-8. PubMed Central PMCID: PMC313233.
[40] Nuijten PJ, van Asten FJ, Gaastra W, van der Zeijst BA. Structural and functional analysis of two Campylobacter jejuni flagellin genes. J Biol Chem. 1990;265(29):17798-804.
[41] Olive DM, Johny M, Sethi SK. Use of an alkaline phosphatase-labeled synthetic oligonucleotide probe for detection of Campylobacter jejuni and Campylobacter coli. J Clin Microbiol. 1990;28(7):1565-9.
[42] Ouellette M, Gerbaud G, Lambert T, Courvalin P. Acquisition by a Campylobacter-like strain of aphA-1, a kanamycin resistance determinant from members of the family Enterobacteriaceae. Antimicrob Agents Chemother. 1987;31(7):1021-6.
[43] Patton CM, Wachsmuth IK, Evins GM, Kiehlbauch JA, Plikaytis BD, Troup N, Tompkins L, Lior H. Evaluation of 10 methods to distinguish epidemic-associated Campylobacter strains. J Clin Microbiol. 1991;29(4):680-8.
[44] Raschtchian A, Abbott MA, Shaffer M. Cloning and characterization of genes coding for ribosomal RNA inCampylobacter jejuni. Curr Microbiol. 1986;14(6):311–7.
[45] Rashtchian A, Shaffer M. The nucleotide sequences of two tRNA genes from Campylobacter jejuni. Nucleic Acids Res. 1986;14(13):5560.
[46] Ritchie AE, Bryner JH, Foley JW. Role of DNA and bacteriophage in Campylobacter auto-agglutination. J Med Microbiol. 1983;16(3):333-40.
[47] Sagara H, Mochizuki A, Okamura N, Nakaya R. Antimicrobial resistance of Campylobacter jejuni and Campylobacter coli with special reference to plasmid profiles of Japanese clinical isolates. Antimicrob Agents Chemother. 1987;31(5):713-9.
[48] Salama S, Bolton FJ, Hutchinson DN. Improved method for the isolation of Campylobacter jejuni and Campylobacter coli bacteriophages. Lett Appl Microbiol. 1989;8(1):5–7.
[49] Salama SM, Garcia MM, Taylor DE. Differentiation of the subspecies of Campylobacter fetus by genomic sizing. Int J Syst Bacteriol. 1992;42(3):446-50.
[50] Salama SM, Tabor H, Richter M, Taylor DE. Pulsed-field gel electrophoresis for epidemiologic studies of Campylobacter hyointestinalis isolates. J Clin Microbiol. 1992;30(8):1982-4.
[51] Sharp PM, Li WH. The codon Adaptation Index--a measure of directional synonymous codon usage bias, and its potential applications. Nucleic Acids Res. 1987;15(3):1281-95.
[52] Sougakoff W. Nucleotide sequence and distribution of gene tetO encoding tetracycline resistance in Campylobacter coli. FEMS Microbiol Lett. 1987;44(1):153–9.
[53] Taylor DE. Plasmids from Campylobacter. Campylobacter infection in man and animals. Ed. J. P. Butzler. Boca Raton : CRC press, 1984:87-96.
[54] Taylor DE. Plasmid-mediated tetracycline resistance in Campylobacter jejuni: expression in Escherichia coli and identification of homology with streptococcal class M determinant. J Bacteriol. 1986;165(3):1037-9.
[55] Taylor DE, Courvalin P. Mechanisms of antibiotic resistance in Campylobacter species. Antimicrob Agents Chemother. 1988;32(8):1107-12.
[56] Taylor DE, Garner RS, Allan BJ. Characterization of tetracycline resistance plasmids from Campylobacter jejuni and Campylobacter coli. Antimicrob Agents Chemother. 1983;24(6):930-5.
[57] Taylor DE, Hiratsuka K. Use of non-radioactive DNA probes for detection of Campylobacter jejuni and Campylobacter coli in stool specimens. Mol Cell Probes. 1990;4(4):261-71.
[58] Taylor DE, Hiratsuka K, Mueller L. Isolation and characterization of catalase-negative and catalase-weak strains of Campylobacter species, including "Campylobacter upsaliensis," from humans with gastroenteritis. J Clin Microbiol. 1989;27(9):2042-5.
[59] Taylor DE, Johnson WM, Lior H. Cytotoxic and enterotoxic activities of Campylobacter jejuni are not specified by tetracycline resistance plasmids pMAK175 and pUA466. J Clin Microbiol. 1987;25(1):150-1.
[60] Taylor DE, Yan W, Ng LK, Manavathu EK, Courvalin P. Genetic characterization of kanamycin resistance in Campylobacter coli. Ann Inst Pasteur Microbiol. 1988;139(6):665-76.
[61] Tenover FC, Elvrum PM. Detection of two different kanamycin resistance genes in naturally occurring isolates of Campylobacter jejuni and Campylobacter coli. Antimicrob Agents Chemother. 1988;32(8):1170-3. PubMed Central
[62] Tenover FC, Gilbert T, O'Hara P. Nucleotide sequence of a novel kanamycin resistance gene, aphA-7, from Campylobacter jejuni and comparison to other kanamycin phosphotransferase genes. Plasmid. 1989;22(1):52-8.
[63] Thornton SA, Logan SM, Trust TJ, Guerry P. Polynucleotide sequence relationships among flagellin genes of Campylobacter jejuni and Campylobacter coli. Infect Immun. 1990;58(8):2686-9.
[64] Trieu-Cuot P, Gerbaud G, Lambert T, Courvalin P. In vivo transfer of genetic information between gram-positive and gram-negative bacteria. EMBO J. 1985;4(13A):3583-7.
[65] Vieira J, Messing J. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene. 1982;19(3):259-68.
[66] Wang Y. Transformation and antibiotic resistance in Campylobacter species. PhD thesis. Edmonton : Univ. Alberta, 1991.
[67] Wang Y, Taylor DE. Natural transformation in Campylobacter species. J Bacteriol. 1990;172(2):949-55.
[68] Wang Y, Taylor DE. Chloramphenicol resistance in Campylobacter coli: nucleotide sequence, expression, and cloning vector construction. Gene. 1990;94(1):23-8.
[69] Wassenaar TM, Bleumink-Pluym NM, van der Zeijst BA. Inactivation of Campylobacter jejuni flagellin genes by homologous recombination demonstrates that flaA but not flaB is required for invasion. EMBO J. 1991;10(8):2055-61.
[70] Wenman WM, Chai J, Louie TJ, Goudreau C, Lior H, Newell DG, Pearson AD, Taylor DE. Antigenic analysis of Campylobacter flagellar protein and other proteins. J Clin Microbiol. 1985;21(1):108-12.
[71] Zilhao R, Papadopoulou B, Courvalin P. Occurrence of the Campylobacter resistance gene tetO in Enterococcus and Streptococcus spp. Antimicrob Agents Chemother. 1988;32(12):1793-6.
[72] Blaser MJ, Taylor DN, Feldman RA. Epidemiology of Campylobacter jejuni infections. Epidemiol Rev. 1983;5:157-76.
[73] Griffiths PL, Park RW. Campylobacters associated with human diarrhoeal disease. J Appl Bacteriol. 1990;69(3):281-301.
[74] Penner JL. The genus Campylobacter: a decade of progress. Clin Microbiol Rev. 1988;1(2):157-72.
[75] Taylor DE, Hiratsuka K, Ray H, Manavathu EK. Characterization and expression of a cloned tetracycline resistance determinant from Campylobacter jejuni plasmid pUA466. J Bacteriol. 1987;169(7):2984-9.