Biopolym. Cell. 1985; 1(4):183-193.
http://dx.doi.org/10.7124/bc.000180
Структура та функції біополімерів
Кількісне вивчення взаємодії деацильованої тРНК з Р-, А- і Е-сайтами 70S рибосом Escherichia coli
1Семенков Ю. П., 1Макаров Є. М., 1Кириллов С. В.
  1. Ленінградський інститут ядерної фізики ім. Б. П. Константинова АН СРСР
    Гатчина, Ленінградська обл., СРСР

Abstract

Визначено залежності констант асоціації деацильованої тРНКPhe з Р-сайтом рибосом від концентрації іонів Mg2+ і температури; виміряно константу асоціації тРНКPhe з А-сайтом; показано, що із зміною концентрації Mg2+ і температури взаємодія тPHKPhe з Е-сайтом залишається кодоннезалежною. На основі отриманих та опублікованих нами раніше даних побудовано ряди відносної спорідненості аміноацил-тРНК, пептидил-тРНК і деацильованої тРНК до Р-, А- та Е-сайтів 70S рибосоми, а також запропоновано термодинамічно обгрунтовану модель транслокації з використанням Е-сайта як вихідного.
Повний текст: (PDF, російською)

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