Biopolym. Cell. 1985; 1(3):135-140.
Біополімери та регуляція генома
Мікроін’єктування генів. Проблеми їхньої експресії і регуляції
1Морозова Л. М., 1Соломко О. П.
  1. Інститут молекулярної біології і генетики АН УСРС
    Київ, СРСР


Представлено огляд робіт із включення у геном ссавців чужорідних генів. Обговорюються питання, пов’язані з регулюванням експресії цих генів, її стабільністю та успадковуваністю введеної генетичної інформації.


[1] Jaenisch R, Mintz B. Simian virus 40 DNA sequences in DNA of healthy adult mice derived from preimplantation blastocysts injected with viral DNA. Proc Natl Acad Sci U S A. 1974;71(4):1250-4.
[2] Gordon JW, Scangos GA, Plotkin DJ, Barbosa JA, Ruddle FH. Genetic transformation of mouse embryos by microinjection of purified DNA. Proc Natl Acad Sci U S A. 1980;77(12):7380-4.
[3] Gordon JW, Ruddle FH. Integration and stable germ line transmission of genes injected into mouse pronuclei. Science. 1981;214(4526):1244-6.
[4] Wold B, Wigler M, Lacy E, Maniatis T, Silverstein S, Axel R. Introduction and expression of a rabbit beta-globin gene in mouse fibroblasts. Proc Natl Acad Sci U S A. 1979;76(11):5684-8.
[5] Wagner TE, Hoppe PC, Jollick JD, Scholl DR, Hodinka RL, Gault JB. Microinjection of a rabbit beta-globin gene into zygotes and its subsequent expression in adult mice and their offspring. Proc Natl Acad Sci U S A. 1981;78(10):6376-80.
[6] Wagner EF, Mintz B. Transfer of nonselectable genes into mouse teratocarcinoma cells and transcription of the transferred human beta-globin gene. Mol Cell Biol. 1982;2(2):190-8.
[7] Anderson FW, Diacumakos EG. Genetic engineering in mammalian cells. Scientific American. 1981; 245:106-21.
[8] Costantini F, Lacy E. Introduction of a rabbit beta-globin gene into the mouse germ line. Nature. 1981;294(5836):92-4.
[9] Palmiter RD, Chen HY, Brinster RL. Differential regulation of metallothionein-thymidine kinase fusion genes in transgenic mice and their offspring. Cell. 1982;29(2):701-10.
[10] Brinster RL, Chen HY, Warren R, Sarthy A, Palmiter RD. Regulation of metallothionein--thymidine kinase fusion plasmids injected into mouse eggs. Nature. 1982;296(5852):39-42.
[11] Palmiter RD, Brinster RL, Hammer RE, Trumbauer ME, Rosenfeld MG, Birnberg NC, Evans RM. Dramatic growth of mice that develop from eggs microinjected with metallothionein-growth hormone fusion genes. Nature. 1982;300(5893):611-5.
[12] Costantini F, Lacy E. Gene transfer into the mouse germ-line. J Cell Physiol Suppl. 1982;1:219-26.
[13] Anderson WF, Killos L, Sanders-Haigh L, Kretschmer PJ, Diacumakos EG. Replication and expression of thymidine kinase and human globin genes microinjected into mouse fibroblasts. Proc Natl Acad Sci U S A. 1980;77(9):5399-403.
[14] Southern EM. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975;98(3):503-17.
[15] Durnam DM, Palmiter RD. Transcriptional regulation of the mouse metallothionein-I gene by heavy metals. J Biol Chem. 1981;256(11):5712-6.
[16] Mayo KE, Palmiter RD. Glucocorticoid regulation of metallothionein-I mRNA synthesis in cultured mouse cells. J Biol Chem. 1981;256(6):2621-4.
[17] Durnam DM, Perrin F, Gannon F, Palmiter RD. Isolation and characterization of the mouse metallothionein-I gene. Proc Natl Acad Sci U S A. 1980;77(11):6511-5.
[18] Beach LR, Palmiter RD. Amplification of the metallothionein-I gene in cadmium-resistant mouse cells. roc Natl Acad Sci U S A. 1981;78(4):2110-4.
[19] Gick GG, McCarty KS Sr. Amplification of the metallothionein-I gene in cadmium- and zinc-resistant Chinese hamster ovary cells. J Biol Chem. 1982;257(15):9049-53.
[20] Pavlakis GN, Hamer DH. Regulation of a metallothionein-growth hormone hybrid gene in bovine papilloma virus. Proc Natl Acad Sci U S A. 1983;80(2):397-401.
[21] Mayo K. E.t Palmiter R. D. Altered regulation of mouse: metallothionein-1 gene following gene amplification or transfection. In: Gene Amplificat. Conf. (Cold Spring Harbor, Oct. 4-7, 1981): Abstracts. N. Y.: Cold Spring Harbor, 1982:67-73.
[22] Chapman AB, Costello MA, Lee F, Ringold GM. Amplification and hormone-regulated expression of a mouse mammary tumor virus-Eco gpt fusion plasmid in mouse 3T6 cells. Mol Cell Biol. 1983;3(8):1421-9.
[23] Kurtz D. Hormonal inducibility of rat a2n-globin genes in transfected mouse cells. Nature, 1981, 291, N 5817, p. 629-631.
[24] Brinster RL, Chen HY, Trumbauer M, Senear AW, Warren R, Palmiter RD. Somatic expression of herpes thymidine kinase in mice following injection of a fusion gene into eggs. Cell. 1981;27(1 Pt 2):223-31.
[25] Hauser H, Gross G, Bruns W, Hochkeppel HK, Mayr U, Collins J. Inducibility of human beta-interferon gene in mouse L-cell clones. Nature. 1982;297(5868):650-4.
[26] Brinster RL, Palmiter RD. Induction of foreign genes in animals. Trends Biochem. Sci., 1982: 7(12):438-440.
[27] McKnight GS, Hammer RE, Kuenzel EA, Brinster RL. Expression of the chicken transferrin gene in transgenic mice. Cell. 1983;34(2):335-41.
[28] Lacy E, Roberts S, Evans EP, Burtenshaw MD, Costantini FD. A foreign beta-globin gene in transgenic mice: integration at abnormal chromosomal positions and expression in inappropriate tissues. Cell. 1983;34(2):343-58.
[29] Jaenisch R, Harbers K, Schnieke A, L?hler J, Chumakov I, J?hner D, Grotkopp D, Hoffmann E. Germline integration of moloney murine leukemia virus at the Mov13 locus leads to recessive lethal mutation and early embryonic death. Cell. 1983;32(1):209-16.
[30] Pollack Y, Stein R, Razin A, Cedar H. Methylation of foreign DNA sequences in eukaryotic cells. Proc Natl Acad Sci U S A. 1980;77(11):6463-7.
[31] Stein R, Gruenbaum Y, Pollack Y, Razin A, Cedar H. Clonal inheritance of the pattern of DNA methylation in mouse cells. Proc Natl Acad Sci U S A. 1982;79(1):61-5.
[32] Waechter DE, Baserga R. Effect of methylation on expression of microinjected genes. Proc Natl Acad Sci U S A. 1982;79(4):1106-10.
[33] Vanyushin B. F. DNA methylation in eukaryotes - In: Macromol. Funct. Coll. (Pushchino, Dec. 9-12, 1980): Abstracts 2 Sov.-Ital. Symp. Moscow, 1982, p. 174-175.
[34] Felsenfeld G, McGhee J. Methylation and gene control. Nature. 1982;296(5858):602-3.
[35] Ovchinnikov IuA, Sverdlov ED, Tsarev SA, Khodkova EM, Monastyrskaia GS. Direct expression of the gene of human leukocyte interferon F in Escherichia coli cells. Dokl Akad Nauk SSSR. 1982;265(1):238-42.
[36] Cline MJ, Stang H, Mercola K, Morse L, Ruprecht R, Brown J, Salser W. Gene transfer in intact animals. Nature. 1980;284(5755):422-5.
[37] Mercola KE, Stang HD, Browne J, Salser W, Cline MJ. Insertion of a new gene of viral origin into bone marrow cells of mice. Science. 1980;208(4447):1033-5.