Biopolym. Cell. 1993; 9(6):41-49.
http://dx.doi.org/10.7124/bc.00037F
Structure and Function of Biopolymers
The aging dependent effects of proteins and mtDNA content in mitochondrial fractions of rat liver
1Orlichenko L. S., 2Beregovskaya N. N., 1Litoshenko A. Ya.
  1. Gerontology Institute of the Academy of Medical Sciences of Ukraine
    67, Vyshgorodska Str., Kyiv, Ukraine, 04114
  2. Science and Engineering Center automated biotechnical systems "SONAR"
    Vladimirskaya Str., 41, Kiev, 252034

Abstract

The contents of mtDNA and proteins encoded by mtDNA and nDNA were determined in liver mitochondrial fractions of rats of different ages (young – 3–4, adult – 6–8, old – 24–26 mo). Three mitochondrial fractions with different sedimentation constants have been varied by content and ratio of mtDNA and proteins as well. The absolute and relative content of the fractions were changed irregularly in liver mass unit with age. Furthemore, it was observed that the content and ratio of mtDNA and proteins changed in each mitochondrial fraction with age. These changes were more pronounced in the old rats as compared with adult ones. The mechanisms of coordination alterations of mitochondrial genome replication and expression are discussed.
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References

[1] Frolkis VV. Aging and the biological capacity of the organism. M: Nauka, 1975. 272 p.
[2] Hansford RG. Bioenergetics in aging. Biochim Biophys Acta. 1983;726(1):41-80.
[3] Litoshenko AY. Aging mitochondria-triggering mechanism of cell aging. Reliability and elementary "events of aging processes of biological objects. Kiev: Naukova Dumka, 1986: 150-60.
[4] Roodyn DB, Wilkie D. The biogenesis of mitochondria. London : Methuen, 1968. 123 p.
[5] Ozernyuk ND. Growth and reproduction of the mitochondria. Moscow: Nauka, 1973. 263 p.
[6] Tomura H, Endo H, Kagawa Y, Ohta S. Novel regulatory enhancer in the nuclear gene of the human mitochondrial ATP synthase beta-subunit. J Biol Chem. 1990;265(12):6525-7.
[7] Skulachev VP. The accumulation of energy in the cell. Moscow: Nauka, 1969. 440 p.
[8] Litoshenko AIa. The trigger of the aging process--in the nucleus or the mitochondria?. Izv Akad Nauk SSSR Biol. 1992;(4):645-8.
[9] Litoshenko AIa. Synthesis of mitochondrial proteins coded in the nucleus and mitochondria of the intact and regenerating liver of rats of various ages. Vopr Med Khim. 1985;31(2):105-10.
[10] Litoshenko AIa. Mitochondrial protein turnover in the liver of rats of different ages. Biull Eksp Biol Med. 1982;94(12):49-51.
[11] Litoshenko AIa. Renewal of mitochondrial DNA in the liver of rats of different ages. Biull Eksp Biol Med. 1984;97(3):299-301.
[12] Tauchi H, Sato T. Hepatic cells of the aged. Liver and Ageng. Amsterdam; New York, Oxford : Elsevier, 1978: 3-20.
[13] Beattie DS. Studies on the biogenesis of mitochondrial protein components in rat liver slices. J Biol Chem. 1968;243(15):4027-33.
[14] Adrianov NV, Blednov IuA, Shuppe NG. Biochemical heterogeneity of mitochondria. Mol Biol (Mosk). 1976;10(5):1042-9.
[15] Petit PX, O'Connor JE, Grunwald D, Brown SC. Analysis of the membrane potential of rat- and mouse-liver mitochondria by flow cytometry and possible applications. Eur J Biochem. 1990;194(2):389-97.
[16] Kitagawa Y, Sugimoto E. Estimation of the in vivo translational activity of rat liver mitochondria without use of an antibiotic. J Biochem. 1980;88(3):689-93.
[17] Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951;193(1):265-75.
[18] Skvirskaya EB, Chepinoga OP. Practicum on nucleoprotein and nucleic acids. M. Vyssh. shk., 1964. 214 p.
[19] Riabinina ZA, Benyush VA. Polyploidy and hypertrophy of cells in the growth and recovery. M.: Medicine, 1973. 208 p.
[20] Posakony JW, England JM, Attardi G. Mitochondrial growth and division during the cell cycle in HeLa cells. J Cell Biol. 1977;74(2):468-91.
[21] Hare JF, Crane FL. Proteins of mitochondrial cristae. Subcell Biochem. 1974; 3(1):1-25.
[22] Azzi A. Mitochondria: the utilization of oxygen for cell life. Experientia. 1984;40(9):901-6.
[23] Massie HR, Colacicco JR, Williams TR. Loss of mitochondrial DNA with aging in the Swedich c strain of Drosophila melanogaster. Age. 1981;(1):42-7.
[24] Huemer RP, Lee KD, Reeves AE, Bickert C. Mitochondrial studies in senescent mice. II. Specific activity, buoyant density, and turnover of mitochondrial DNA. Exp Gerontol. 1971;6(5):327-34.
[25] Novikov NM, Bondarenko YuV. The effect of age on the quantitative changes in the DNA of rat liver mitochondria. Mol. and Physiol. mechanisms of age development. Kiev: Naukova Dumka, 1975: 148-53.
[26] Stocco DM, Hutson JC. Quantitation of mitochondrial DNA and protein in the liver of Fischer 344 rats during aging. J Gerontol. 1978;33(6):802-9.
[27] Cortopassi GA, Arnheim N. Detection of a specific mitochondrial DNA deletion in tissues of older humans. Nucleic Acids Res. 1990;18(23):6927-33.
[28] Linnane AW, Baumer A, Maxwell RJ, Preston H, Zhang CF, Marzuki S. Mitochondrial gene mutation: the ageing process and degenerative diseases. Biochem Int. 1990;22(6):1067-76.
[29] Wallace DC. Diseases of the mitochondrial DNA. Annu Rev Biochem. 1992;61:1175-212.
[30] Neifach SA. Role of mitochondria in cell heredity. Mol. mitochondrial genetics. M . : Nauka, 1977: 8-18.
[31] Asano K, Amagase S, Matsuura ET, Yamagishi H. Changes in the rat liver mitochondrial DNA upon aging. Mech Ageing Dev. 1991;60(3):275-84.
[32] Asano K, Nakamura M, Asano A, Sato T, Tauchi H. Quantitation of changes in mitochondrial DNA during aging and regeneration of rat liver using non-radioactive DNA probes. Mech Ageing Dev. 1992;64(1-2):85-98.
[33] Saul RL, Gee P, Ames BN. Free radical, DNA damage, and aging. Modern Biol. Theor Aging. New York: Raven press, 1987: 113-29.