Biopolym. Cell. 1992; 8(1):101-107.
http://dx.doi.org/10.7124/bc.000316
Structure and Function of Biopolymers
Interaction of eukaryotic aminoacyl-tRNA synthetases with ribosomes
1Sana Sara, 2Mozuraitis R. J., 1Kharchenko O. V., 1Ivanov L. L., 1Turkovskaya G. V., 2Martinkus Z. P., 1Kovalenko M. I., 1El'skaya A. V.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  2. Kaunas Medical Academy
    Mizkevicha Str. 9, Kaunas, Lithuania, 44307

Abstract

The activities of eukaryotic aminoacyl-tRNA synthetases associated with polyribosomes have been determined under the different levels of protein biosynthesis. A decrease of some aminoacyl-tRNA synthetase activities in the fraction of polyribosomes as well as in the fractions of free and membrane-bound ribosomes has been observed during experimental myocardial ischemia, which results in the reduce of the translational level in cell-free system. The activity of leucyl-tRNA synthetase in the fraction of cow mammary gland polyribosome has been increase under lactation. The stimulating effect of 40S and 60S ribosomal subunits and 80S ribosomes on the activity of rabbit liver leucyl-tRNA synthetase as well as on the positive cooperativity of tRNA-binding sites has been revealed.
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