Biopolym. Cell. 1992; 8(1):82-88.
http://dx.doi.org/10.7124/bc.000314
Structure and Function of Biopolymers
A study of side-reactions in H-phosphonate DNA fragments synthesis
1Dubey I. Ya., 1Ljapina T. V., 1Fedoryak D. M.
  1. Institute of Bioorganic Chemistry and Petrochemistry, NAS of Ukraine
    1, Murmans'ka Str., Kyiv, Ukraine, 02094

Abstract

Possibe side-reactions during the internucleotide condensation in H-phosphonate oligonucleotide synthesis, such as OH-component and P–H bonds acylatiorr and base modification, and their kinetics in presence of some bases have been studied. Stable modifications of heterocyclic bases by pivaloyl chloride were observed. It has been shown that P–H bonds acylation leads to oligonucleotide chain cleavage at acylated sites. The side-reactions rates were found to be several times lower in quinoline than in more basic pyridine.
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