Biopolym. Cell. 1991; 7(5):62-65.
Cell Biology
Somatic hybridization in genus Brassica: hybrids between rapeseed (Brassica napus L.) and black mustard (Brassica nigra L.)
1Sahno L. A., 1Cherep N. N., 1Skarzhynskaya M. V., 1Gleba Yu. Yu.
  1. Institute of Cell Biology and Genetic Engineering, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR

Abstract

By using of the method of somatic hybridization with double inactivation of hypocotyl protoplasts of B. napus and mesophyll protoplasts of B. nigra were obtained 3 clones of hybrid plants. Genetic novelity of regenerants was shown by study of morphology, physiology, isozymes (esterase, amylase, aspartataminotransferase), chloroplast DNAs. Obtained hybrid plants may be treated by practical selection.

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