Biopolym. Cell. 1991; 7(5):33-36.
http://dx.doi.org/10.7124/bc.0002EF
Structure and Function of Biopolymers
The immunochemical approach for studies of structure tyrosyl-tRNA synthetase from bovine liver
1Ribkinska T. A., 1Kornelyuk A. I., 2Beresten S. F., 1Matsuka G. Kh.
  1. Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR
  2. Engelhardt Institute of Molecular Biology, Academy of Sciences of the USSR
    Moscow, USSR

Abstract

The antigenic determinant for mAbs T3 against tyrosyl-tRNA synthetase from bovine liver has been localized outside of the active site of enzyme. This epitope also remains for the active truncated enzyme form (dimer of 39 kDa subunits) product by limited endogenous proteolysis. The tyrosyl-tRNA synthetases of higher eukaryotes (bovine liver, rabbit liver, human placenta) have the common antigenic determinant, which lacks in tyrosyl-tRNA synthetases from prokaryots (E. coli, Th. thermophilus) and from yeast as revealed by immunoblot analysis.
Full text: (PDF, in Russian)

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