Biopolym. Cell. 1991; 7(4):82-91.
http://dx.doi.org/10.7124/bc.0002E6
Cell Biology
Changes of the genetic constitution of the tomato plants by «gamma-fusion» of Lycopersicon esculentum Mill, and Lycopersicon peruvianum var. dentatum dun. protoplasts
1Ratushnyak Ya. I., 1Piven N. M., 1Latypov S. A., 1Samoylov A. M., 1Zavgorodnyaya A. V., 1Gleba Yu. Yu.
  1. Institute of Cell Biology and Genetic Engineering, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR

Abstract

Asymmetric somatic hybrids between Lycopersicon esculentum Mill, (plastome chlorophyll-deficient PL alb 1 mutant) and Lycopersicon peruvianum var. dentatum Dun. (lines 3767, 3772) were obtained by mesophyll protoplast fusion. The protoplasts ot the wild tomato plants were inactivated by the lethal dosage of gamma-irradiation (100 and 200 Gy). The colonies capable of greening were selected. The observed restoration of the photosynthetic activity was because of wild type of chloroplasts were transferred from L. peruvianum var. dentatum. The hybridization efficiency dependent on irradiation dosage but never exceeded 6%. The hybrid obtained were classified into six groups on the bases of their ploidity, morphological (bush form and size, stem tomentosity, leaf form, flower quantity, pistil position, fruit size and colour) and biochemical (esterase, aspartateaminotransferase and acid phosphatase isozymes, restriction pattern of chloroplast and mitochondrial DNAs) traits. The variability of the hybrid plants of these groups dependent upon irradiation dosage and the L. peruvianum var. dentatum genotype.
Full text: (PDF, in Russian)

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