Biopolym. Cell. 1991; 7(4):61-65.
Cell Biology
In vitro induced morphogenesis of walnut
- Institute of Cell Biology and Genetic Engineering, Academy of Sciences of the Ukrainian SSR
Kiev, USSR
Abstract
The ability of apical axillary buds of 15–30 and 1–3 years old walnut trees to morphogenesis in vitro was studied. Adventitious shoot formation was induced on MS- media containing IBA (0,01 mg/1) and 6-BAP (1 mg/l) after 12 weeks of cultivation (including two subcultivations after 2 and 6 weeks). Propagated shoots were pretreated in liquid MS-medium with IBA (1; 5; 10 mg/1) and sucrose (60 g/l) during 6 days in dark. Rooting of the walnut shoots was achieved on hormone free MS-medium (macro-salts were dissolved 1/4) with agar.
Full text: (PDF, in Russian)
References
[1]
Rybin VA methods of vegetative propagation of walnut. Kishinev: Shtiintsa, 1961. 29 p.
[2]
Tsurkan IP. Growing grafted walnut planting material in Moldova. Achievements in the fruit and nursery management NRB MSSR. Chisinau Maps Moldovenyaska. 1978:208-213.
[3]
Strela TE. New biotechnology walnut grafting. Kiev: Naukova Dumka, 1987. 59.
[4]
Chalupa V. Vegetativni mnozeni brizy (Betula pendula Roth.), dubu (Quercus robur L.) a jasanu (Fraxinus escelsior L.) in vitro. Pr. vulhm. 1983;62:179-94.
[5]
Pua Eng-Chong, Chong Calvin. Regulation of in vitro shoot and root regeneration in Macspur apple by sorbitol (Z)-glucitol) and related carbon sources. J Amer Soc Hort Sci. 1985; 110(5):705-9.
[6]
Driver JA, Kuniyuki AH. In vitro propagation of Paradox wal nut rootstock. Hort Sci. 1984; 19(4):507-9.
[7]
Meynier V. Mise en culture in vitro de meristeme de Noyers hybrioes. C r Acad Sci C. 1985; 301(5):261-4.
[8]
Wood BW. In vitro proliferation of pecan. Hort Sci. 1982; 17(6):890-1.
[9]
Liu S, Han B. In vitro propagation of walnut (Juglans regia L.). Acta Agr Univ. Pekinesis. 1986; 12(2):143-8.
[10]
Tulecke W, McGranahan G. Somatic embryogenesis and plant regeneration from cotyledons of walnut, Juglans regia L. Plant Sci. 1985;40(1):57–63.
[11]
Felaliev A. Morphogenesis Juglans regia in vitro. Ukr Bot Zh. 1990;47(3):85-7.
[12]
Boltivets VS, Piven NM. Induced embryoidogenesis in the culture tissue of Juglans regia L. Fiziologiia i biokhimiia kul't rasteniy. 1990; 22(4):409-13
[13]
Murashige T, Skoog F. A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Physiol Plant. 1962;15(3):473–97.
[14]
Jay-allemand C, Cornu D, Macheix JJ, Chanteloup G. Caractérisation du rajeunissement du Noyer (Juglans sp.) par une étude spectrophotométrique globale du contenu polyphénolique. Ann For Sci. 1987;44(3):303–14.
[15]
Felaliev A. Physiological basis of micropropagation of walnut in vitro: Author. dis. ... Kand. biol. nauk. Dushanbe, 1990. 20 p.
