Biopolym. Cell. 1991; 7(3):60-65.
http://dx.doi.org/10.7124/bc.0002D1
Structure and Function of Biopolymers
Study on yeast phenylalanine tRNA anti-codon arm interaction with small ribosomal subunits of rabbit liver
1Koval'chuk O. V., 1Potapov A. P.
  1. Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR

Abstract

The 15-nucleotide analog of the yeast tRNAPheanticodon arm appears to bind cooperatively to two equal in affinity sites of poly(U)-programmed 40S ribosome as well as intact tRNAPhe. However the fragment affinity for the ribosome is higher. This is mostly contributed by higher cooperative coefficient for the anticodon arm-ribosome complex formation. Cooperativity coefficients are 4 for tRNAPhe and 47 for its anticodon arm, meanwhile, if cooperativity coefficient is not considered, association constants are 1,2 · 107 M–1 (tRNAPhe) and 0,5 · 107 M–1 (the arm) for each site at 2 °C and 20 mM Mg2+ concentration The correct codon-anticodon pairing is shown to play the key role in cooperativity origin. Template independent binding of tRNA to small ribosomal subunits is revealed, meanwhile, anticodon arm is not able to bind to 40S ribosomes in the absence of template.
Full text: (PDF, in Russian)

References

[1] Rodnina MV, El'skaya AV, Semenkov YuP, Kirillov SV. Number of tRNA binding sites on 80 S ribosomes and their subunits. FEBS Lett. 1988;231(1):71-4.
[2] Potapov AP. A stereospecific mechanism for the aminoacyl-tRNA selection at the ribosome. FEBS Lett. 1982;146(1):5-8.
[3] Potapov AP. Mechanism of the stereospecific stabilization of codon-anticodon complexes in ribosomes during translation. Zh Obshch Biol. 1985;46(1):63-77.
[4] Rose SJ 3rd, Lowary PT, Uhlenbeck OC. Binding of yeast tRNAPhe anticodon arm to Escherichia coli 30 S ribosomes. J Mol Biol. 1983;167(1):103-17.
[5] Nekhay S. A., Saminsky E. M. Binding of yeast tRNAPhe with anticodon arm to Escherichia coli 305 Subunits and 70S ribosomes. Biopolym Cell. 1989; 5(2):62-9.
[6] Falvey AK, Staehelin T. Structure and function of mammalian ribosomes. I. Isolation and characterization of active liver ribosomal subunits. J Mol Biol. 1970;53(1):1-19.
[7] Kirillov SV, Makhno VI, Semenkov IuP. Effect of the molecular weight of polyuridylic acid and the presence of ribosomal protein S1 on the stability of the complex of transport RNA with small ribosomal subunits. Dokl Akad Nauk SSSR. 1976;229(2):488-91.
[8] Potapov AP, Soldatkin KA, Soldatkin AP, El'skaya AV. The role of a template sugar-phosphate backbone in the ribosomal decoding mechanism. Comparative study of poly(U) and poly(dT) template activity. J Mol Biol. 1988;203(4):885-93.
[9] Maxam AM, Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977;74(2):560-4.
[10] Nirenberg M, Leder P. RNA codewords and protein synthesis. The effect of trinucleotides upon the binding of sRNA to ribosomes. Science. 1964;145(3639):1399-407.
[11] Rodnina MV, El'skaya AV, Semenkov YP, Kirillov SV. Interaction of tRNA with the A and P sites of rabbit-liver 80S ribosomes and their 40S subunits. Eur J Biochem. 1989;185(3):563-8.
[12] Ackers GK, Shea MA, Smith FR. Free energy coupling within macromolecules. The chemical work of ligand binding at the individual sites in co-operative systems. J Mol Biol. 1983;170(1):223-42.
[13] Metzler D. Biochemistry: the chemical reactions of living cells. Elsevier, 1977; 1164 p
[14] Dahlquist FW. The quantitative interpretation of maximum in Scatchard plots. FEBS Lett. 1974;49(2):267-8.