Biopolym. Cell. 1990; 6(5):98-102.
http://dx.doi.org/10.7124/bc.000295
Cell Biology
Effect of blood serum and plasma in rats with regenerating liver on proliferation of normal and transformed hepatocytes in the primary culture
1Kononenko L. I., 1Alekseeva T. A., 1Ivashchenko Yu. D., 1Bykorez A. I.
  1. R. E. Kavetsky Institute for Oncology Problems, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR

Abstract

Blood serum of intact rats slightly modifies proliferative activity of normal hepatocytes in the primary culture. At the same time, the serum from intact and hepatectomized rats effectively inhibits the proliferation of hepatocytes stimulated by epidermal growth factor (EGF) and insulin. Transformed hepatocytes from the liver tumours are less sensitive to mitogenic effects of EGF and insulin. Serum from intact and hepatectomized rats inhibits EGF-stimulated DNA-synthesis in transformed hepatocytes, though serum alone slightly influences the hepatocytes proliferative rate. The same inhibitory activity has been found in the rat blood plasma.
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