Biopolym. Cell. 1990; 6(3):77-80.
http://dx.doi.org/10.7124/bc.000271
Gene-Engineering Biotechnology
Genetic transformation of pea plants mediated by Agrobacterium tumefaciens
1Zubko E. I., 1Kuchuk N. V., 2Tumanova L. G., 2Vikonskaya N. A., 1Gleba Yu. Yu.
  1. N. G. Kholodny Institute of Botany, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR
  2. Institute of Ecological Genetics, Academy of Sciences of the Moldavian SSR
    Kishinev, USSR

Abstract

The actively regenerating lines of pea (Pisum sativum L.) on the hormone-free B5 medium are selected after cocultivation of shoot and leave segments with the «shooty» mutant of Agrobacterium tumcfaciens pGV 2206. These lines retain the regeneration ability at least for 18 months. Normalized but phenotypically different shoots are obtained. DNA-DNA hybridization analysis has revealed the presence of the structural NPT II gene in the BamH1-digested total DNA isolated from pea regenerants. Transgenic shoots grafted on the normal pea plants gave both flowers and seeds.
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