Isolation of seryl-tRNA synthetase from the animal liver by proximate method

Gudzera, O. I.; Sidorik, L. L.; Zolotukhina, I. V.; Tukalo, M. A.; Matsuka, G. Kh.

doi:10.7124/bc.000264

Biopolym. Cell. 1990; 6(2):105-107.

http://dx.doi.org/10.7124/bc.000264

Short Communications

Isolation of seryl-tRNA synthetase from the animal liver by proximate method

1Gudzera O. I., 1Sidorik L. L., 1Zolotukhina I. V., 1Tukalo M. A., 1Matsuka G. Kh.

Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
Kiev, USSR

Abstract

A proximate method to isolate seryl-tRNA synthetatse from the animal liver is described. It includes polyethylene glycole 6000 fractionation, chromatography on DEAE-Toyopearl 650 M, and chromatography on the FPLC system of Mono S and Mono Q sorbents. A yield of the purified enzyme was about 600 ng per 1 kg of liver. The enzyme is a structural dimer (oc2 type) with molecular weight of 120 kDa.

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