Biopolym. Cell. 1989; 5(6):87-92.
Gene-Engineering Biotechnology
A fast method for cloning of specific cDNA. Cloning of human prolactin cDNA
1Zolotukhin S. B., 1Markelova E. Yu., 1Panasenko G. V., 1Naidenov V. G., 1Shved A. D., 2Sautin Yu. Yu., 3Patsko Ya. V.
- Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
Kiev, USSR - Institute of Neurosurgery, Ministry of Public Health of the Ukrainian SSR
Kiev, USSR - Lvov Branch of A. V. Palladin Institute of Biochemistry, Academy of Sciences of the Ukrainian SSR
Lvov, USSR
Abstract
A method allowing fast and effective cloning of particular cDNA is described. Pairs of synthetic oligonucleotides-primers are used for polymerize chain reaction mediated selective amplification of prolactine cDNTA synthesized on human pituitary poly (A)-mRNA. Sequence of one of the recombinant plasmid insert revealed a full-length pro-lactin cDNA, flanked by the synthetic primers.
Full text: (PDF, in Russian)
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