Biopolym. Cell. 1989; 5(6):46-52.
http://dx.doi.org/10.7124/bc.0000F6
Structure and Function of Biopolymers
Detection of directions of dna alkylation with ethyleneimine derivatives and isolation of modified bases
1Patskovsky Yu. V., 1Voloshchuk T. P., 1Potopalsky A. I.
  1. Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR

Abstract

Alkylation of purine bases with ethyleneimine, monoaziridinediethylphosphate and thio-phosphoamide leads to formation of ·1-, 3- and 9-alkylderivatives of adenine, 6-alkyIaini-nopurine and 1-, 7- and 9-alkylderivatives of guaninc. 1- and 7-alkylguanine, 1- and 3-alkyladenine, 6-alkylaminopurine were obtained from the acidic hydrolysates of alkylated DNA. Dependence of the physicochemical properties of alkylated DNA on the direction of purine bases alkylation is discussed.
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