Biopolym. Cell. 1989; 5(5):64-70.
Structure and Function of Biopolymers
Some properties of the reaction between polynucleotides and thiophosphamide
1Patskovsky Yu. V., 1Voloshchuk T. P., 1Potopalsky A. I.
  1. Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR

Abstract

The alkylation of ribonucleosides and polynucleotides with ethylene imine, monoazindine, diethyl phosphate and antitumour agent thiophosphamide has been shown to change usually nucleobases residues. The preferential centres of the aklylation are nitrogen atoms in the positions 7 of guanosine, 1 of adenosine and 3 of pyrimidine nucleosides. The rate of DNA alkylation increases with a decrease of the ionic strength and pH and depends on the conformation and molecular mass of the molecules.

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